TY - JOUR
T1 - Components in Lentinus edodes mushroom with anti-biofilm activity directed against bacteria involved in caries and gingivitis
AU - Papetti, Adele
AU - Signoretto, Caterina
AU - Spratt, David A.
AU - Pratten, Jonathan
AU - Lingström, Peter
AU - Zaura, Egija
AU - Ofek, Itzhak
AU - Wilson, Michael
AU - Pruzzo, Carla
AU - Gazzani, Gabriella
N1 - Publisher Copyright:
© 2018 The Royal Society of Chemistry.
PY - 2018/6
Y1 - 2018/6
N2 - The present study investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine at 5 mg mL-1 both in the biofilm inhibition (BI 50%) and biofilm disaggregation tests (BD 20%). Considering A. naeslundii, BI values close to 80% were registered for oxalic acid at 1 mg mL-1 and 2 mg mL-1 and BD 50% for quinic acid at 3 mg mL-1. A weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometimes negatively affected by the presence of the other compounds.
AB - The present study investigated the compounds present in the low molecular mass fraction of Lentinus edodes mushroom (shiitake) extract and their anti-virulence activity against oral pathogens (reference and clinical Streptococcus mutans, Actinomyces naeslundii, and Prevotella intermedia strains). Oxalic, succinic, and quinic acids, and adenine, inosine, and uridine were identified by HPLC-DAD-ESI-MS/MS. Their anti-biofilm production and preformed biofilm disaggregation activities were studied using commercial standard compounds at different concentrations. As regards S. mutans, the highest activity was shown by adenine at 5 mg mL-1 both in the biofilm inhibition (BI 50%) and biofilm disaggregation tests (BD 20%). Considering A. naeslundii, BI values close to 80% were registered for oxalic acid at 1 mg mL-1 and 2 mg mL-1 and BD 50% for quinic acid at 3 mg mL-1. A weaker activity was found against P. intermedia. Furthermore, different mixtures of the commercial standards were tested showing that the activity of a compound can be strongly and sometimes negatively affected by the presence of the other compounds.
UR - http://www.scopus.com/inward/record.url?scp=85048966394&partnerID=8YFLogxK
U2 - 10.1039/c7fo01727h
DO - 10.1039/c7fo01727h
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AN - SCOPUS:85048966394
SN - 2042-6496
VL - 9
SP - 3489
EP - 3499
JO - Food and Function
JF - Food and Function
IS - 6
ER -