@inbook{206425344c334185bf38f79c5d707944,
title = "Complex Formation Among TGF-β Receptors in Live Cell Membranes Measured by Patch-FRAP",
abstract = "Signaling by receptors from the transforming growth factor-β (TGF-β) superfamily plays critical roles in multiple physiological and pathological processes. Their signaling requires complex formation between type I and type II receptors with Ser/Thr kinase activity, whereby the type II receptor phosphorylates and activates the relevant type I receptor(s), which transduces downstream signaling. It is therefore important to study complex formation among receptors from this family. In the current chapter, we use the type I (ALK5) and type II TGF-β receptors (TβRI and TβRII) as an example for measuring complex formation among cell-surface receptors in live cells by patch-FRAP, a variation of fluorescence recovery after photobleaching (FRAP).",
keywords = "FRAP, Lateral diffusion, Patch-FRAP, Photobleaching, Receptor complex formation, Transforming growth factor-β receptors",
author = "Szil{\'a}gyi, {Szabina Sz{\'o}fia} and Orit Gutman and Henis, {Yoav I.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2277-3_3",
language = "אנגלית",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "23--34",
booktitle = "Methods in Molecular Biology",
}