Complex Formation Among TGF-β Receptors in Live Cell Membranes Measured by Patch-FRAP

Szabina Szófia Szilágyi, Orit Gutman, Yoav I. Henis*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Signaling by receptors from the transforming growth factor-β (TGF-β) superfamily plays critical roles in multiple physiological and pathological processes. Their signaling requires complex formation between type I and type II receptors with Ser/Thr kinase activity, whereby the type II receptor phosphorylates and activates the relevant type I receptor(s), which transduces downstream signaling. It is therefore important to study complex formation among receptors from this family. In the current chapter, we use the type I (ALK5) and type II TGF-β receptors (TβRI and TβRII) as an example for measuring complex formation among cell-surface receptors in live cells by patch-FRAP, a variation of fluorescence recovery after photobleaching (FRAP).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • FRAP
  • Lateral diffusion
  • Patch-FRAP
  • Photobleaching
  • Receptor complex formation
  • Transforming growth factor-β receptors


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