TY - JOUR
T1 - Comparison of matched formalin-fixed paraffin embedded and fresh frozen meningioma tissue reveals bias in proteomic profiles
AU - Schoffman, Hanan
AU - Levin, Yishai
AU - Itzhaki-Alfia, Ayelet
AU - Tselekovits, Lea
AU - Gonen, Lior
AU - Vainer, Gilad Wolf
AU - Hout-Siloni, Goni
AU - Barshack, Iris
AU - Cohen, Zvi R.
AU - Margalit, Nevo
AU - Shahar, Tal
N1 - Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/11
Y1 - 2022/11
N2 - Tissue biopsies are most commonly archived in a paraffin block following tissue fixation with formaldehyde (FFPE) or as fresh frozen tissue (FFT). While both methods preserve biological samples, little is known about how they affect the quantifiable proteome. We performed a ‘bottom-up’ proteomic analysis (N = 20) of short and long-term archived FFPE surgical samples of human meningiomas and compared them to matched FFT specimens. FFT facilitated a similar number of proteins assigned by MetaMorpheus compared with matched FFPE specimens (5378 vs. 5338 proteins, respectively (p = 0.053), regardless of archival time. However, marked differences in the proteome composition were apparent between FFPE and FFT specimens. Twenty-three percent of FFPE-derived peptides and 8% of FFT-derived peptides contained at least one chemical modification. Methylation and formylation were most prominent in FFPE-derived peptides (36% and 17% of modified FFPE peptides, respectively) while, most of phosphorylation and iron modifications appeared in FFT-derived peptides (p < 0.001). A mean 14% (± 2.9) of peptides identified in FFPE contained at least one modified Lysine residue. Importantly, larger proteins were significantly overrepresented in FFT specimens, while FFPE specimens were enriched with smaller proteins.
AB - Tissue biopsies are most commonly archived in a paraffin block following tissue fixation with formaldehyde (FFPE) or as fresh frozen tissue (FFT). While both methods preserve biological samples, little is known about how they affect the quantifiable proteome. We performed a ‘bottom-up’ proteomic analysis (N = 20) of short and long-term archived FFPE surgical samples of human meningiomas and compared them to matched FFT specimens. FFT facilitated a similar number of proteins assigned by MetaMorpheus compared with matched FFPE specimens (5378 vs. 5338 proteins, respectively (p = 0.053), regardless of archival time. However, marked differences in the proteome composition were apparent between FFPE and FFT specimens. Twenty-three percent of FFPE-derived peptides and 8% of FFT-derived peptides contained at least one chemical modification. Methylation and formylation were most prominent in FFPE-derived peptides (36% and 17% of modified FFPE peptides, respectively) while, most of phosphorylation and iron modifications appeared in FFT-derived peptides (p < 0.001). A mean 14% (± 2.9) of peptides identified in FFPE contained at least one modified Lysine residue. Importantly, larger proteins were significantly overrepresented in FFT specimens, while FFPE specimens were enriched with smaller proteins.
KW - formalin-fixed paraffin-embedded tissue, mass spectrometry, meningioma, proteomics
KW - fresh frozen tissue
UR - http://www.scopus.com/inward/record.url?scp=85138242320&partnerID=8YFLogxK
U2 - 10.1002/pmic.202200085
DO - 10.1002/pmic.202200085
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C2 - 36098096
AN - SCOPUS:85138242320
SN - 1615-9853
VL - 22
JO - Proteomics
JF - Proteomics
IS - 21
M1 - 2200085
ER -