Comparative study of antithrombin III-protease complex metabolism by fibroblasts and vascular endothelial cells

Naphtali Savion, Nahid Farzame

Research output: Contribution to journalArticlepeer-review

Abstract

125I-labeled human antithrombin III (125I-AT III)-protease complexes are specifically bound to both cultured human skin fibroblast (HSF) cells and adult bovine aortic endothelial (ABAE) cells; however, there is a significant difference in the rate and degree of metabolism of the complexes by these two cell types. HSF cells appear to internalize the complexes at a rate of about 2.5 pmole/1×106 cells/h and subsequently degrade them at a rate of 0.6 pmole/1×106 cells/h. ABAE cells internalize and degrade the complexes at rates approximately 100 and 30 times lower, respectively. Neither cell type interacts with free 125I-AT III but only with its combined form with either thrombin or trypsin. These data indicate the major role of HSF cells in the removal of AT III protease complexes from extravascular spaces in the body, in contrast to the inert vascular surface with regard to AT III.protease complexes provided by the vascular endothelium.

Original languageEnglish
Pages (from-to)459-470
Number of pages12
JournalThrombosis Research
Volume41
Issue number4
DOIs
StatePublished - 15 Feb 1986

Keywords

  • Antithrombin III
  • Fibroblasts
  • Vascular Endothelial Cells

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