TY - JOUR
T1 - Common fixation-permeabilization methods cause artifactual localization of a type II transmembrane protein
AU - Benyair, Ron
AU - Lederkremer, Gerardo Z.
N1 - Publisher Copyright:
© The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved.For permissions, please e-mail: [email protected].
PY - 2016/12/1
Y1 - 2016/12/1
N2 - We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.
AB - We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.
KW - Golgi
KW - fixation
KW - mannosidase
KW - permeabilization
KW - transmembrane protein
KW - vesicles
UR - http://www.scopus.com/inward/record.url?scp=85041257843&partnerID=8YFLogxK
U2 - 10.1093/jmicro/dfw035
DO - 10.1093/jmicro/dfw035
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C2 - 27587511
AN - SCOPUS:85041257843
SN - 2050-5698
VL - 65
SP - 517
EP - 521
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
IS - 6
ER -