Combined phage typing and amperometric detection of released enzymatic activity for the specific identification and quantification of bacteria

T. Neufeld, A. Schwartz-Mittelmann, D. Biran, E. Z. Ron, J. Rishpon

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we describe a novel electrochemical method for the rapid identification and quantification of pathogenic and polluting bacteria. The design incorporates a bacteriophage, a virus that recognizes, infects, and lyses only one bacterial species among mixed populations, thereby releasing intracellular enzymes that can be monitored by the amperometic measurement of enzymatic activity. As a model system, we used virulent phage typing and cell- marker enzyme activity (β-D-galactosidase), a combination that is specific for the bacterial strain Escherichia coli (K-12, MG1655). Filtration and preincubation before infecting the bacteria with the phage enabled amperometric detection at a wide range of concentrations, reaching as low as 1 colony-forming unit/100 mL within 6-8 h. In principle, this electrochemical method can be applied to any type of bacterium-phage combination by measuring the enzymatic marker released by the lyric cycle of a specific phage.

Original languageEnglish
Pages (from-to)580-585
Number of pages6
JournalAnalytical Chemistry
Volume75
Issue number3
DOIs
StatePublished - 1 Feb 2003

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