Collagen-like peptide as a matrix for enzyme immobilization in electrochemical biosensors

Miri Yemini*, Peng Xu, David L. Kaplan, Judith Rishpon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

An efficient enzyme immobilization technique is described in which well-organized collagen peptide monolayers are used as a scaffold upon which horseradish peroxidase is anchored on gold electrode surfaces. The resulting electrode presents reproducible amperometric responses at an applied potential of -0.1 V with a range of linearity for peroxides. These results suggest that triple helical collagen can be used for specific immobilization of HRP with advantages of low price, simplicity and biocompatibility. The results are significant for the control of biomolecular self-assembly in the intrinsic electric devices.

Original languageEnglish
Pages (from-to)2049-2054
Number of pages6
JournalElectroanalysis
Volume18
Issue number21
DOIs
StatePublished - 2006

Keywords

  • Biosensor
  • Collagen-like peptide
  • Electrode
  • HRP
  • Hydrogen peroxide

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