Collagen composition and content-dependent contrast in porcine annulus fibrosus achieved by using double quantum and magnetization transfer filtered UTE MRI

Uzi Eliav, Michal E. Komlosh, Peter J. Basser, Gil Navon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Purpose To test the potential of combining double quantum and magnetization transfer filtered ultra-short echo time (DQF-MT-UTE) MRI to obtain information about the macromolecular composition and characteristics of connective tissues. Methods A DQF-MT-UTE pulse sequence was implemented on a 14.1 T AVANCE III Bruker spectrometer equipped with a Bruker micro2.5-imaging gradient system to obtain images of porcine annulus fibrosus. Results The DQF-MT-UTE MRI of the annulus fibrosus of porcine intervertebral disc, where the creation time of the double quantum coherence filtering (DQF) was on a time scale appropriate for excitation of macromolecules, showed stronger signal from the outer layers of the disc than from the inner layers closer to the nucleus pulposus. Similarly, spectroscopic studies showed the same trend in the efficiency of the magnetization transfer (MT) from collagen to water. Conclusion DQF-MT filtered UTE MRI of the annulus fibrosus provides new contrast parameters that depend on the concentration of the collagen and on the rate and efficiency of MT of its protons to water. The latter parameters appear to be different for collagen types I and II in the annulus fibrosus.

Original languageEnglish
Pages (from-to)388-393
Number of pages6
JournalMagnetic Resonance in Medicine
Volume71
Issue number1
DOIs
StatePublished - Jan 2014

Funding

FundersFunder number
National Institute of Child Health and Human DevelopmentZIAHD008756

    Keywords

    • annulus fibrosus
    • collagen type I
    • collagen type II
    • double quantum filter
    • interverterbral disc
    • magnetization transfer
    • ultra-short TE

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