Cloning and expression of a rat brain GABA transporter

John Guastella, Nathan Nelson, Hannah Nelson, Linda Czyzyk, Shoshi Keynan, May C. Miedel, Norman Davidson, Henry A. Lester, Baruch I. Kanner

Research output: Contribution to journalArticlepeer-review

Abstract

A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter-γ-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [ 3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.

Original languageEnglish
Pages (from-to)1303-1306
Number of pages4
JournalScience
Volume249
Issue number4974
DOIs
StatePublished - 1990
Externally publishedYes

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