Cloning and expression of a glycine transporter from mouse brain

Qing Rong Liu, Hannah Nelson, Sreekala Mandiyan, Beatriz López-Corcuera, Nathan Nelson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated a cDNA clone from a mouse brain library encoding the glycine transporter (GLYT). Xenopus oocytes injected with a synthetic mRNA accumulated [3H]glycine to levels of up to 80-fold above control values. The uptake was specific for glycine and dependent on the presence of Na+ and Cl- in the medium. The cDNA sequence predicts a highly hydrophobic protein of 633 amino acids with 12 potential transmembrane helices. The predicted amino acid sequence has 40-45% identity to the GABA, noradrenaline, serotonin and dopamine transporters. This implies that all of these neurotransmitter, transporters may have evolved from a common ancestral gene that diverged into the GABA, glycine and catecholamine subfamilies at nearly the same time.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalFEBS Letters
Volume305
Issue number2
DOIs
StatePublished - 29 Jun 1992
Externally publishedYes

Keywords

  • Expression
  • Glycine, Cloning
  • Neurotransmitter
  • Transport

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