Cloning and characterization of the human homolog of mouse Jak2

Ilan Dalal, Enrico Arpaia, Harjit Dadi, Shaila Kulkarni, Jerami Squire, Chaim M. Roifman

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the Jak family play a critical role in signal transduction mediated by cytokine and hormone receptors. In this study, we report the cloning and characterization of human Jak2. The predicted amino acid sequence shows 91% homology to the described murine Jak2, but with a significant difference in the extreme C-terminal sequence. Using the human cDNA as a probe, we localized the gene for human Jak2 to chromosome 9p23-24. Human Jak2 mRNA is highly expressed in the spleen, lymph nodes, and peripheral blood lymphocytes (PBLs). A polyclonal antibody raised against the unique C- terminus of human Jak2 was used to characterize Jak2 protein. Levels of Jak2 protein expression increased significantly in mitogen- and anti-IgM- stimulated B cells and to a lesser degree in activated T cells. In addition, high levels of Jak2 protein were detected in pre-B leukemia cells.

Original languageEnglish
Pages (from-to)844-851
Number of pages8
JournalBlood
Volume91
Issue number3
DOIs
StatePublished - 1 Feb 1998

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