Cloning and characterization of a Myxococcus xanthus cytochrome P-450 hydroxylase required for biosynthesis of the polyketide antibiotic TA

Yossi Paitan, Elisha Orr, Eliora Z. Ron, Eugene Rosenberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The antibiotic TA, a complex macrocyclic polyketide of Myxococcus xanthus, is produced, like many other polyketides, through successive condensations of acetate by a type I polyketide synthase (PKS) mechanism. The chemical structure of this antibiotic and the mechanism by which it is synthesized indicate the need for several post-modification steps, such as a specific hydroxylation at C-20. Previous studies have shown that several genes, essential for TA biosynthesis, are clustered in a region of at least 36 kb, which was subsequently cloned and analyzed. In this study, we report the analysis of a DNA fragment, containing a specific cytochrome P-450 hydroxylase, presumably responsible for the sole non-PKS hydroxylation at position C-20. Functional analysis of the cytochrome P-450 hydroxylase gene through specific gene disruption confirms that it is essential for the production of an active TA molecule.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalGene
Volume228
Issue number1-2
DOIs
StatePublished - 4 Mar 1999

Keywords

  • Cytochrome P-450 hydroxylase
  • Hydroxylation
  • PKS
  • Polyketides

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