Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

Daniel Chamovitz, Iris Pecker, Gerhard Sandmann, Peter Böger, Joseph Hirschberg

Research output: Contribution to journalArticlepeer-review

Abstract

The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

Original languageEnglish
Pages (from-to)482-486
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume45
Issue number5
DOIs
StatePublished - May 1990
Externally publishedYes

Keywords

  • Cyanobacteria
  • Gene Cloning
  • Herbicide Resistance
  • Norflurazon
  • Phytoene Dehydrogenase (= Phytoene Desaturase)

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