Clinical modeling of T cell vaccination against autoimmune disease in rats. Selection of antigen-specific T cell using a mitogen

F. Mor; A. W. Lohse; N. Karin; I. R. Cohen

doi:10.1172/JCI114610

Clinical modeling of T cell vaccination against autoimmune disease in rats. Selection of antigen-specific T cell using a mitogen

F. Mor, A. W. Lohse, N. Karin, I. R. Cohen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Effective T cell vaccination against experimental autoimmune diseases involves treatment with activated, autoimmune T lymphocytes. The present study was undertaken to learn whether antigen-specific T cells present in low frequency could be selected in vitro without using the specific antigen. The rat models of adjuvant arthritis and experimental autoimmune encephalomyelitis were investigated using proliferation assays and limiting dilution techniques to quantify the changes in reactivity of a heterogenous population of lymphocytes to the relevant antigen. Stimulation with concanavalin A for 2 d and then culture in IL-2-containing medium led to a substantial increase in the activity and frequency of the specific autoimmune T cells.

Original language	English
Pages (from-to)	1594-1598
Number of pages	5
Journal	Journal of Clinical Investigation
Volume	85
Issue number	5
DOIs	https://doi.org/10.1172/JCI114610
State	Published - 1990
Externally published	Yes

Keywords

T cell activation
T cell vaccination
autoimmune disease
concanavalin A
interleukin 2

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10.1172/JCI114610

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abstract = "Effective T cell vaccination against experimental autoimmune diseases involves treatment with activated, autoimmune T lymphocytes. The present study was undertaken to learn whether antigen-specific T cells present in low frequency could be selected in vitro without using the specific antigen. The rat models of adjuvant arthritis and experimental autoimmune encephalomyelitis were investigated using proliferation assays and limiting dilution techniques to quantify the changes in reactivity of a heterogenous population of lymphocytes to the relevant antigen. Stimulation with concanavalin A for 2 d and then culture in IL-2-containing medium led to a substantial increase in the activity and frequency of the specific autoimmune T cells.",

keywords = "T cell activation, T cell vaccination, autoimmune disease, concanavalin A, interleukin 2",

author = "F. Mor and Lohse, {A. W.} and N. Karin and Cohen, {I. R.}",

year = "1990",

doi = "10.1172/JCI114610",

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AU - Cohen, I. R.

PY - 1990

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AB - Effective T cell vaccination against experimental autoimmune diseases involves treatment with activated, autoimmune T lymphocytes. The present study was undertaken to learn whether antigen-specific T cells present in low frequency could be selected in vitro without using the specific antigen. The rat models of adjuvant arthritis and experimental autoimmune encephalomyelitis were investigated using proliferation assays and limiting dilution techniques to quantify the changes in reactivity of a heterogenous population of lymphocytes to the relevant antigen. Stimulation with concanavalin A for 2 d and then culture in IL-2-containing medium led to a substantial increase in the activity and frequency of the specific autoimmune T cells.

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KW - T cell vaccination

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Clinical modeling of T cell vaccination against autoimmune disease in rats. Selection of antigen-specific T cell using a mitogen

Abstract

Keywords

UN SDGs

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