Climate chamber for environmentally controlled laboratory airflow experiments

Nurit Even-Tzur*, Uri Zaretsky, Orly Grinberg, Tomer Davidovich, Yoel Kloog, Michael Wolf, David Elad

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Climate chambers have been widely used in in vitro and in vivo studies which require controlled environmental temperature and humidity conditions. This article describes a new desktop climate chamber that was developed for application of respiratory airflows on cultured nasal epithelial cells (NEC) under controlled temperature and humidity conditions. Flow experiments were performed by connecting the climate chamber to an airflow generator via a flow chamber with cultured NEC. Experiments at two controlled climate conditions, 25°C and 40% relative humidity (RH) and 37°C and 80%RH, were conducted to study mucin secretion from the cultures inresponse to the flow. The new climate chamber is a relatively simple and inexpensive apparatus which can easily be connected to any flow system for climate controlled flow experiments. This chamber can be easily adjusted to various in vitro experiments, as well as to clinical studies with animals or human subjects which require controlled climate conditions.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalTechnology and Health Care
Volume18
Issue number3
DOIs
StatePublished - 2010

Keywords

  • Air-liquid interface (ALI)
  • environmental chamber
  • mucin secretion
  • nasal epithelial cells
  • respiratory airflow

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