Claudin-5 Expression in In Vitro Models of the Blood–Brain Barrier

Itzik Cooper, Katayun Cohen-Kashi-Malina, Vivian I. Teichberg*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

17 Scopus citations

Abstract

Claudins are transmembrane proteins that form the backbone of the tight junctions (TJs) at the blood–brain barrier (BBB). TJs are cellular structures that physically obstruct the inter-endothelial space and restrict the paracellular diffusion of blood-borne substances from the peripheral circulation into the CNS. TJs are also dynamic structures that rapidly respond to external signals that produce changes in BBB permeability. We focus here on the biochemical and immunohistochemical properties of claudin-5 as expressed in three in vitro models of the BBB, and show that the contact co-culture of endothelial cells with glial cells significantly increases claudin-5 expression.

Original languageEnglish
Title of host publicationClaudins
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages347-354
Number of pages8
ISBN (Print)9781617791840
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume762
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Funding

FundersFunder number
Weizmann-Negri Fund
Israel Science Foundation

    Keywords

    • BBB
    • Claudin-5
    • Endothelial cells
    • Glia
    • Immunocytochemistry
    • In vitro model
    • Tight junctions
    • Western blot

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