TY - JOUR
T1 - Clathrin-mediated endocytosis cooperates with bulk endocytosis to generate vesicles
AU - Arpino, Gianvito
AU - Somasundaram, Agila
AU - Shin, Wonchul
AU - Ge, Lihao
AU - Villareal, Seth
AU - Chan, Chung Yu
AU - Ashery, Uri
AU - Shupliakov, Oleg
AU - Taraska, Justin W.
AU - Wu, Ling Gang
N1 - Publisher Copyright:
© 2022
PY - 2022/2/18
Y1 - 2022/2/18
N2 - Clathrin-mediated endocytosis, the most prominent endocytic mode, is thought to be generated primarily from relatively flat patches of the plasma membrane. By employing conventional and platinum replica electron microscopy and super-resolution STED microscopy in neuroendocrine chromaffin cells, we found that large Ω-shaped or dome-shaped plasma membrane invaginations, previously thought of as the precursor of bulk endocytosis, are primary sites for clathrin-coated pit generation after depolarization. Clathrin-coated pits are more densely packed at invaginations rather than flat membranes, suggesting that invaginations are preferred sites for clathrin-coated pit formation, likely because their positive curvature facilitates coated-pit formation. Thus, clathrin-mediated endocytosis closely collaborates with bulk endocytosis to enhance endocytic capacity in active secretory cells. This direct collaboration between two classically independent endocytic pathways is of broad importance given the central role of both clathrin-mediated endocytosis and bulk endocytosis in neurons, endocrine cells, immune cells, and many other cell types throughout the body.
AB - Clathrin-mediated endocytosis, the most prominent endocytic mode, is thought to be generated primarily from relatively flat patches of the plasma membrane. By employing conventional and platinum replica electron microscopy and super-resolution STED microscopy in neuroendocrine chromaffin cells, we found that large Ω-shaped or dome-shaped plasma membrane invaginations, previously thought of as the precursor of bulk endocytosis, are primary sites for clathrin-coated pit generation after depolarization. Clathrin-coated pits are more densely packed at invaginations rather than flat membranes, suggesting that invaginations are preferred sites for clathrin-coated pit formation, likely because their positive curvature facilitates coated-pit formation. Thus, clathrin-mediated endocytosis closely collaborates with bulk endocytosis to enhance endocytic capacity in active secretory cells. This direct collaboration between two classically independent endocytic pathways is of broad importance given the central role of both clathrin-mediated endocytosis and bulk endocytosis in neurons, endocrine cells, immune cells, and many other cell types throughout the body.
KW - Biological sciences
KW - Cell biology
KW - Endocrine regulation
KW - Neuroscience
UR - http://www.scopus.com/inward/record.url?scp=85124212810&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2022.103809
DO - 10.1016/j.isci.2022.103809
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C2 - 35198874
AN - SCOPUS:85124212810
SN - 2589-0042
VL - 25
JO - iScience
JF - iScience
IS - 2
M1 - 103809
ER -