CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments

R. Bhagya Lakshmi; Pinaki Nayak; Linoy Raz; Apurba Sarkar; Akshay Saroha; Pratibha Kumari; Vishnu M. Nair; Delvin P. Kombarakkaran; S. Sajana; M. G. Sanusha; Sarit S. Agasti; Raja Paul; Uri Ben-David; Tapas K. Manna

doi:10.1038/s44319-024-00106-9

CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments

R. Bhagya Lakshmi, Pinaki Nayak, Linoy Raz, Apurba Sarkar, Akshay Saroha, Pratibha Kumari, Vishnu M. Nair, Delvin P. Kombarakkaran, S. Sajana, M. G. Sanusha, Sarit S. Agasti, Raja Paul, Uri Ben-David, Tapas K. Manna^*

^*Corresponding author for this work

Human Molecular Genetics Biochemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

(Figure presented.) Microtubule +TIP protein CKAP5 stabilizes CENP-E kinesin at the kinetochores and is essential for chromosome congression. CKAP5 depletion results in hyper-stable kinetochore-attached microtubules and increases recruitment of PP1, thereby causing CENP-E removal from kinetochores. CKAP5 stabilizes CENP-E at aligned kinetochores. CKAP5 depletion induces hyper-stability of kinetochore-attached microtubule. CKAP5 depletion promotes merotelic attachment errors. CKAP5 maintains optimum levels of PP1 on attached kinetochores. CKAP5 mediated regulation on PP1 and microtubule stability ensure CENP-E stabilization at kinetochores.

Original language	English
Pages (from-to)	1909-1935
Number of pages	27
Journal	EMBO Reports
Volume	25
Issue number	4
DOIs	https://doi.org/10.1038/s44319-024-00106-9
State	Published - 12 Apr 2024

Funding

Funders	Funder number
Council of Scientific and Industrial Research, India
Department of Science and Technology, Ministry of Science and Technology, India
European Research Council
Department of Biotechnology, Ministry of Science and Technology, India
Novocure
IACS, Kolkata, India
Horizon 2020 Framework Programme	945674
Israel Science Foundation	1805/21
Israel Cancer Association	20230018
University of California, San Diego	T422A

Keywords

CENP-E
CKAP5
Kinetochore
Microtubule
PP1

Access to Document

10.1038/s44319-024-00106-9

Cite this

@article{c4c2f0bb8ec547f4a091b04c20e067fb,

title = "CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments",

abstract = "(Figure presented.) Microtubule +TIP protein CKAP5 stabilizes CENP-E kinesin at the kinetochores and is essential for chromosome congression. CKAP5 depletion results in hyper-stable kinetochore-attached microtubules and increases recruitment of PP1, thereby causing CENP-E removal from kinetochores. CKAP5 stabilizes CENP-E at aligned kinetochores. CKAP5 depletion induces hyper-stability of kinetochore-attached microtubule. CKAP5 depletion promotes merotelic attachment errors. CKAP5 maintains optimum levels of PP1 on attached kinetochores. CKAP5 mediated regulation on PP1 and microtubule stability ensure CENP-E stabilization at kinetochores.",

keywords = "CENP-E, CKAP5, Kinetochore, Microtubule, PP1",

author = "Lakshmi, \{R. Bhagya\} and Pinaki Nayak and Linoy Raz and Apurba Sarkar and Akshay Saroha and Pratibha Kumari and Nair, \{Vishnu M.\} and Kombarakkaran, \{Delvin P.\} and S. Sajana and Sanusha, \{M. G.\} and Agasti, \{Sarit S.\} and Raja Paul and Uri Ben-David and Manna, \{Tapas K.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = apr,

day = "12",

doi = "10.1038/s44319-024-00106-9",

language = "אנגלית",

volume = "25",

pages = "1909--1935",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "4",

}

TY - JOUR

T1 - CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments

AU - Lakshmi, R. Bhagya

AU - Nayak, Pinaki

AU - Raz, Linoy

AU - Sarkar, Apurba

AU - Saroha, Akshay

AU - Kumari, Pratibha

AU - Nair, Vishnu M.

AU - Kombarakkaran, Delvin P.

AU - Sajana, S.

AU - Sanusha, M. G.

AU - Agasti, Sarit S.

AU - Paul, Raja

AU - Ben-David, Uri

AU - Manna, Tapas K.

PY - 2024/4/12

Y1 - 2024/4/12

N2 - (Figure presented.) Microtubule +TIP protein CKAP5 stabilizes CENP-E kinesin at the kinetochores and is essential for chromosome congression. CKAP5 depletion results in hyper-stable kinetochore-attached microtubules and increases recruitment of PP1, thereby causing CENP-E removal from kinetochores. CKAP5 stabilizes CENP-E at aligned kinetochores. CKAP5 depletion induces hyper-stability of kinetochore-attached microtubule. CKAP5 depletion promotes merotelic attachment errors. CKAP5 maintains optimum levels of PP1 on attached kinetochores. CKAP5 mediated regulation on PP1 and microtubule stability ensure CENP-E stabilization at kinetochores.

AB - (Figure presented.) Microtubule +TIP protein CKAP5 stabilizes CENP-E kinesin at the kinetochores and is essential for chromosome congression. CKAP5 depletion results in hyper-stable kinetochore-attached microtubules and increases recruitment of PP1, thereby causing CENP-E removal from kinetochores. CKAP5 stabilizes CENP-E at aligned kinetochores. CKAP5 depletion induces hyper-stability of kinetochore-attached microtubule. CKAP5 depletion promotes merotelic attachment errors. CKAP5 maintains optimum levels of PP1 on attached kinetochores. CKAP5 mediated regulation on PP1 and microtubule stability ensure CENP-E stabilization at kinetochores.

KW - CENP-E

KW - CKAP5

KW - Kinetochore

KW - Microtubule

KW - PP1

UR - http://www.scopus.com/inward/record.url?scp=85186206778&partnerID=8YFLogxK

U2 - 10.1038/s44319-024-00106-9

DO - 10.1038/s44319-024-00106-9

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 38424231

AN - SCOPUS:85186206778

SN - 1469-221X

VL - 25

SP - 1909

EP - 1935

JO - EMBO Reports

JF - EMBO Reports

IS - 4

ER -

CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this