CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments

R. Bhagya Lakshmi, Pinaki Nayak, Linoy Raz, Apurba Sarkar, Akshay Saroha, Pratibha Kumari, Vishnu M. Nair, Delvin P. Kombarakkaran, S. Sajana, M. G. Sanusha, Sarit S. Agasti, Raja Paul, Uri Ben-David, Tapas K. Manna*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

(Figure presented.) Microtubule +TIP protein CKAP5 stabilizes CENP-E kinesin at the kinetochores and is essential for chromosome congression. CKAP5 depletion results in hyper-stable kinetochore-attached microtubules and increases recruitment of PP1, thereby causing CENP-E removal from kinetochores. CKAP5 stabilizes CENP-E at aligned kinetochores. CKAP5 depletion induces hyper-stability of kinetochore-attached microtubule. CKAP5 depletion promotes merotelic attachment errors. CKAP5 maintains optimum levels of PP1 on attached kinetochores. CKAP5 mediated regulation on PP1 and microtubule stability ensure CENP-E stabilization at kinetochores.

Original languageEnglish
Pages (from-to)1909-1935
Number of pages27
JournalEMBO Reports
Volume25
Issue number4
DOIs
StatePublished - 12 Apr 2024

Keywords

  • CENP-E
  • CKAP5
  • Kinetochore
  • Microtubule
  • PP1

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