Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting

R. Brandsen; M. Frusic-Zlotkin; H. Lyubimov; F. Yunes; B. Michel; A. Tamir; Y. Milner; S. Brenner

doi:10.1016/S0190-9622(97)70324-4

Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting

R. Brandsen
, M. Frusic-Zlotkin
, H. Lyubimov
, F. Yunes
, B. Michel
, A. Tamir
, Y. Milner
, S. Brenner^*

^*Corresponding author for this work

Tel Aviv Sourasky Medical Center

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Background: Patients with pemphigus vulgaris (PV) are genetically linked to two alleles of the HLA subgroup, and circulating antibodies were found in first-degree relatives of these patients, thus showing genetic predisposition. Objective: Our purpose was to determine the occurrence of circulating true PV-IgG in patients' relatives. Methods: Circulating PV-IgG was determined in 21 first-degree relatives of 12 patients with PV by indirect immunofluorescence on monkey esophagus, carcinoma A431 cultures, and Western immunoblotting. Direct immunofluorescence was performed on skin biopsy specimens of 20 relatives. Results: Circulating PV-IgG was detected in 15 relatives (71%) by all methods tested. Good correlation was found between immunoblot reactivity and immunofluorescence. Of the 15 'positive' relatives, only five showed fixation of IgG to epidermal cells in vivo. Conclusion: The permeability of the epidermis or epidermal cell reactivity in vivo probably controls the expression of disease in patients' relatives.

Original language	English
Pages (from-to)	44-52
Number of pages	9
Journal	Journal of the American Academy of Dermatology
Volume	36
Issue number	1
DOIs	https://doi.org/10.1016/S0190-9622(97)70324-4
State	Published - 1997
Externally published	Yes

Funding

Funders
Israel Ministry of Absorption
Center of Absorption in Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/S0190-9622(97)70324-4

Cite this

Brandsen, R., Frusic-Zlotkin, M., Lyubimov, H., Yunes, F., Michel, B., Tamir, A., Milner, Y., & Brenner, S. (1997). Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting. Journal of the American Academy of Dermatology, 36(1), 44-52. https://doi.org/10.1016/S0190-9622(97)70324-4

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title = "Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting",

abstract = "Background: Patients with pemphigus vulgaris (PV) are genetically linked to two alleles of the HLA subgroup, and circulating antibodies were found in first-degree relatives of these patients, thus showing genetic predisposition. Objective: Our purpose was to determine the occurrence of circulating true PV-IgG in patients' relatives. Methods: Circulating PV-IgG was determined in 21 first-degree relatives of 12 patients with PV by indirect immunofluorescence on monkey esophagus, carcinoma A431 cultures, and Western immunoblotting. Direct immunofluorescence was performed on skin biopsy specimens of 20 relatives. Results: Circulating PV-IgG was detected in 15 relatives (71\%) by all methods tested. Good correlation was found between immunoblot reactivity and immunofluorescence. Of the 15 'positive' relatives, only five showed fixation of IgG to epidermal cells in vivo. Conclusion: The permeability of the epidermis or epidermal cell reactivity in vivo probably controls the expression of disease in patients' relatives.",

author = "R. Brandsen and M. Frusic-Zlotkin and H. Lyubimov and F. Yunes and B. Michel and A. Tamir and Y. Milner and S. Brenner",

note = "Funding Information: *Department of Pathology, University of Liverpool, Liverpool, t Department of Cardiac Medicine, Cardiothoracic Institute, London, London, U.K., \textasciitilde{}Department of Pathology, People's Hospital of the Tibetan Autonomous Region of the People's Republic of China, Lhasa, §Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing, People's Republic of China and [IDepartment of Cardiology, Postgraduate Institute for Medical Education and Research, Chandigarh, India.",

year = "1997",

doi = "10.1016/S0190-9622(97)70324-4",

language = "אנגלית",

volume = "36",

pages = "44--52",

journal = "Journal of the American Academy of Dermatology",

issn = "0190-9622",

publisher = "Elsevier Inc.",

number = "1",

}

Brandsen, R, Frusic-Zlotkin, M, Lyubimov, H, Yunes, F, Michel, B, Tamir, A, Milner, Y & Brenner, S 1997, 'Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting', Journal of the American Academy of Dermatology, vol. 36, no. 1, pp. 44-52. https://doi.org/10.1016/S0190-9622(97)70324-4

TY - JOUR

T1 - Circulating pemphigus IgG in families of patients with pemphigus

T2 - Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting

AU - Brandsen, R.

AU - Frusic-Zlotkin, M.

AU - Lyubimov, H.

AU - Yunes, F.

AU - Michel, B.

AU - Tamir, A.

AU - Milner, Y.

AU - Brenner, S.

N1 - Funding Information: *Department of Pathology, University of Liverpool, Liverpool, t Department of Cardiac Medicine, Cardiothoracic Institute, London, London, U.K., ~Department of Pathology, People's Hospital of the Tibetan Autonomous Region of the People's Republic of China, Lhasa, §Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing, People's Republic of China and [IDepartment of Cardiology, Postgraduate Institute for Medical Education and Research, Chandigarh, India.

PY - 1997

Y1 - 1997

N2 - Background: Patients with pemphigus vulgaris (PV) are genetically linked to two alleles of the HLA subgroup, and circulating antibodies were found in first-degree relatives of these patients, thus showing genetic predisposition. Objective: Our purpose was to determine the occurrence of circulating true PV-IgG in patients' relatives. Methods: Circulating PV-IgG was determined in 21 first-degree relatives of 12 patients with PV by indirect immunofluorescence on monkey esophagus, carcinoma A431 cultures, and Western immunoblotting. Direct immunofluorescence was performed on skin biopsy specimens of 20 relatives. Results: Circulating PV-IgG was detected in 15 relatives (71%) by all methods tested. Good correlation was found between immunoblot reactivity and immunofluorescence. Of the 15 'positive' relatives, only five showed fixation of IgG to epidermal cells in vivo. Conclusion: The permeability of the epidermis or epidermal cell reactivity in vivo probably controls the expression of disease in patients' relatives.

AB - Background: Patients with pemphigus vulgaris (PV) are genetically linked to two alleles of the HLA subgroup, and circulating antibodies were found in first-degree relatives of these patients, thus showing genetic predisposition. Objective: Our purpose was to determine the occurrence of circulating true PV-IgG in patients' relatives. Methods: Circulating PV-IgG was determined in 21 first-degree relatives of 12 patients with PV by indirect immunofluorescence on monkey esophagus, carcinoma A431 cultures, and Western immunoblotting. Direct immunofluorescence was performed on skin biopsy specimens of 20 relatives. Results: Circulating PV-IgG was detected in 15 relatives (71%) by all methods tested. Good correlation was found between immunoblot reactivity and immunofluorescence. Of the 15 'positive' relatives, only five showed fixation of IgG to epidermal cells in vivo. Conclusion: The permeability of the epidermis or epidermal cell reactivity in vivo probably controls the expression of disease in patients' relatives.

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SN - 0190-9622

VL - 36

SP - 44

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JO - Journal of the American Academy of Dermatology

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IS - 1

ER -

Circulating pemphigus IgG in families of patients with pemphigus: Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting

Abstract

Funding

UN SDGs

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