TY - JOUR
T1 - Circulating pemphigus IgG in families of patients with pemphigus
T2 - Comparison of indirect immunofluorescence, direct immunofluorescence, and immunoblotting
AU - Brandsen, R.
AU - Frusic-Zlotkin, M.
AU - Lyubimov, H.
AU - Yunes, F.
AU - Michel, B.
AU - Tamir, A.
AU - Milner, Y.
AU - Brenner, S.
N1 - Funding Information:
*Department of Pathology, University of Liverpool, Liverpool, t Department of Cardiac Medicine, Cardiothoracic Institute, London, London, U.K., ~Department of Pathology, People's Hospital of the Tibetan Autonomous Region of the People's Republic of China, Lhasa, §Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing, People's Republic of China and [IDepartment of Cardiology, Postgraduate Institute for Medical Education and Research, Chandigarh, India.
PY - 1997
Y1 - 1997
N2 - Background: Patients with pemphigus vulgaris (PV) are genetically linked to two alleles of the HLA subgroup, and circulating antibodies were found in first-degree relatives of these patients, thus showing genetic predisposition. Objective: Our purpose was to determine the occurrence of circulating true PV-IgG in patients' relatives. Methods: Circulating PV-IgG was determined in 21 first-degree relatives of 12 patients with PV by indirect immunofluorescence on monkey esophagus, carcinoma A431 cultures, and Western immunoblotting. Direct immunofluorescence was performed on skin biopsy specimens of 20 relatives. Results: Circulating PV-IgG was detected in 15 relatives (71%) by all methods tested. Good correlation was found between immunoblot reactivity and immunofluorescence. Of the 15 'positive' relatives, only five showed fixation of IgG to epidermal cells in vivo. Conclusion: The permeability of the epidermis or epidermal cell reactivity in vivo probably controls the expression of disease in patients' relatives.
AB - Background: Patients with pemphigus vulgaris (PV) are genetically linked to two alleles of the HLA subgroup, and circulating antibodies were found in first-degree relatives of these patients, thus showing genetic predisposition. Objective: Our purpose was to determine the occurrence of circulating true PV-IgG in patients' relatives. Methods: Circulating PV-IgG was determined in 21 first-degree relatives of 12 patients with PV by indirect immunofluorescence on monkey esophagus, carcinoma A431 cultures, and Western immunoblotting. Direct immunofluorescence was performed on skin biopsy specimens of 20 relatives. Results: Circulating PV-IgG was detected in 15 relatives (71%) by all methods tested. Good correlation was found between immunoblot reactivity and immunofluorescence. Of the 15 'positive' relatives, only five showed fixation of IgG to epidermal cells in vivo. Conclusion: The permeability of the epidermis or epidermal cell reactivity in vivo probably controls the expression of disease in patients' relatives.
UR - http://www.scopus.com/inward/record.url?scp=0031036766&partnerID=8YFLogxK
U2 - 10.1016/S0190-9622(97)70324-4
DO - 10.1016/S0190-9622(97)70324-4
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AN - SCOPUS:0031036766
SN - 0190-9622
VL - 36
SP - 44
EP - 52
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 1
ER -
