Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE

K. Jovanova-Nesic; D. Koruga; D. Kojic; V. Kostic; L. Rakic; Y. Shoenfeld

doi:10.1111/j.1749-6632.2009.04658.x

Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE

K. Jovanova-Nesic^*, D. Koruga, D. Kojic, V. Kostic, L. Rakic, Y. Shoenfeld

^*Corresponding author for this work

Clinical Departments

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

9 Scopus citations

Abstract

Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.

Original language	English
Title of host publication	Contemporary Challenges in Autoimmunity
Publisher	Blackwell Publishing Inc.
Pages	75-82
Number of pages	8
ISBN (Print)	9781573317627
DOIs	https://doi.org/10.1111/j.1749-6632.2009.04658.x
State	Published - Sep 2009

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1173
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

AFM topography
Choroid plexus
Cx43
EAE
GJ

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10.1111/j.1749-6632.2009.04658.x

Cite this

Jovanova-Nesic, K., Koruga, D., Kojic, D., Kostic, V., Rakic, L., & Shoenfeld, Y. (2009). Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE. In Contemporary Challenges in Autoimmunity (pp. 75-82). (Annals of the New York Academy of Sciences; Vol. 1173). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04658.x

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title = "Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE",

abstract = "Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.",

keywords = "AFM topography, Choroid plexus, Cx43, EAE, GJ",

author = "K. Jovanova-Nesic and D. Koruga and D. Kojic and V. Kostic and L. Rakic and Y. Shoenfeld",

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Jovanova-Nesic, K, Koruga, D, Kojic, D, Kostic, V, Rakic, L & Shoenfeld, Y 2009, Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE. in Contemporary Challenges in Autoimmunity. Annals of the New York Academy of Sciences, vol. 1173, Blackwell Publishing Inc., pp. 75-82. https://doi.org/10.1111/j.1749-6632.2009.04658.x

Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE. / Jovanova-Nesic, K.; Koruga, D.; Kojic, D. et al.
Contemporary Challenges in Autoimmunity. Blackwell Publishing Inc., 2009. p. 75-82 (Annals of the New York Academy of Sciences; Vol. 1173).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Jovanova-Nesic, K.

AU - Koruga, D.

AU - Kojic, D.

AU - Kostic, V.

AU - Rakic, L.

AU - Shoenfeld, Y.

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N2 - Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.

AB - Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.

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Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE

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