Choroid plexus connexin 43 expression and gap junction flexibility are associated with clinical features of acute EAE

K. Jovanova-Nesic*, D. Koruga, D. Kojic, V. Kostic, L. Rakic, Y. Shoenfeld

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Experimental autoimmune encephalomyelitis (EAE) induced by ventricular injection of antimyelin oligodendrocyte antibodies in DA rats showed severe clinical signs 4 to 5 days after injection. Immunocytochemically, connexin 43 (Cx43) expression increased in the choroid plexus and in the subventricular and subgranular zones of the hippocampus during the development of acute EAE, and decreased after the beginning of the remission phase of the disease. Quantitative computing analysis showed a significantly increased Cx43 expression in the choroid plexus at the peak of the disease. Plaque-pattern expression of the Cx43 in the choroid plexus (CP) of acute EAE correlated with the increased docking and coupling of the Cx43 hemichannels revealed by atomic force microscopy (AFM). The inner diameter of the gap junction (GJ) channels decreased in the CP of acute EAE, measured by AFM. Cell structure conformational changes showed influences the channels' flexibility in acute EAE.

Original languageEnglish
Title of host publicationContemporary Challenges in Autoimmunity
PublisherBlackwell Publishing Inc.
Pages75-82
Number of pages8
ISBN (Print)9781573317627
DOIs
StatePublished - Sep 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1173
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • AFM topography
  • Choroid plexus
  • Cx43
  • EAE
  • GJ

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