Chemically modified nylons as supports for enzyme immobilization - III. Polyacrylamide-nylon grafts

Linear polyacrylamide bearing 5 mol percent acylhydrazide functional groups was grafted on the surface of chemically modified spun-bonded nylon fabric sheets and nylon fibers. The grafting reaction involved dibromoisocyanide functional groups on the solid polyamide and some of the acylhydrazide groups on the acrylamide polymer, to form five membered heterocyles of the 1,3,4-oxadiazole type. The acylhydrazide groups on polyacrylamidenylon could be utilized directly for the coupling of enzymes by the azide method or converted into other types of chemically reactive groups. Polyacrylamide-nylon derivatives exhibited higher protein binding capacity and higher specific activity and temperature stability of the bound protein than ungrafted nylon supports.