TY - CHAP
T1 - Characterizing Rare Dormant and Cycling Lineages Using the Watermelon System
AU - Handly-Santana, Abram
AU - Oren, Yaara
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - Barcode-based lineage tracing approaches enable molecular characterization of clonal cell families. Barcodes that are expressed as mRNA can be used to deconvolve lineage identity from single-cell RNA sequencing transcriptional data. Here we describe the Watermelon system, which facilitates the simultaneous tracing of lineage, transcriptional, and proliferative state at a single cell level.
AB - Barcode-based lineage tracing approaches enable molecular characterization of clonal cell families. Barcodes that are expressed as mRNA can be used to deconvolve lineage identity from single-cell RNA sequencing transcriptional data. Here we describe the Watermelon system, which facilitates the simultaneous tracing of lineage, transcriptional, and proliferative state at a single cell level.
KW - Expressed barcodes
KW - Lineage tracing
KW - Proliferation tracking
KW - Single-cell RNA sequencing (scRNA-seq)
UR - http://www.scopus.com/inward/record.url?scp=85199381689&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-3882-8_12
DO - 10.1007/978-1-0716-3882-8_12
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C2 - 39037657
AN - SCOPUS:85199381689
T3 - Methods in Molecular Biology
SP - 165
EP - 175
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -