Characterization of IgM molecules in light-chain deficient variants of a B-cell tumor

Mahmoud Taya*, Efrat Rabinovich, Joseph Haimovich

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Characterization of a membrane-IgM-negative variant cell line derived from the murine B-cell line 38C-13 revealed the absence of light chains and the presence of polypeptides with an apparent molecular size of 18 kDa and 14 kDa, previously denoted ω and i{dotless} and characteristic of pre-B cells. These polypeptides assemble with the μ chains into complexes with apparent molecular sizes of about 100 kDa and 200 kDa. It has been previously shown that light-chain-deficient variants of the 38C cell line undergo 'secondary' light chain rearrangements. It is suggested, therefore, that complexes of μ and the 'surrogate' light chains ω and i{dotless} play a role in this process. As these complexes do not reach the cell surface we would like to propose that the mechanism of secondary rearrangement is intracellularly controlled.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalImmunology Letters
Volume33
Issue number2
DOIs
StatePublished - Jul 1992

Funding

FundersFunder number
Israel Cancer Research Fund

    Keywords

    • 'Surrogate' light chain
    • IgM assembly
    • Pre B

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