Characterization of glucose transport system in keratinocytes: Insulin and IGF-1 differentially affect specific transporters

Shlomzion Shen, Efrat Wertheimer, Sanford R. Sampson, Tamar Tennenbaum

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.

Original languageEnglish
Pages (from-to)949-954
Number of pages6
JournalJournal of Investigative Dermatology
Volume115
Issue number6
DOIs
StatePublished - 2000

Funding

FundersFunder number
Smokeless Tobacco Research Council
Sorrell Foundation
Juvenile Diabetes Research Foundation International
Israel Academy of Sciences and Humanities
Israel Science Foundation

    Keywords

    • Glucose transport
    • Glucose transporters
    • Growth factors
    • Insulin
    • Keratinocytes
    • Skin

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