@article{c1f01f3ce1c64a588c2be545d9c5dc12,
title = "Characterization of central melatonin receptors using 125I-melatonin",
abstract = "The binding of 125I-melatonin, a potent analog of melatonin, to rat brain synaptosomal preparations was investigated. 125I-melatonin bound with high affinity (Kd = 38 nM) to a single class of sites (Bmax = 81 fmol mg protein). Kinetic studies indicated that binding was time-dependent and reversible. Specific 125I-melatonin binding was inhibited by melatonin, and was unaffected by other structurally related compounds including serotonin. Binding of 125I-melatonin was greatly reduced if the synaptosomal preparations were pretreated by heat or trypsin but was unaffected by freeze-thawing. These results suggest that 125I-melatonin may serve as a valuable probe for studying melatonin receptors.",
keywords = "Melatonin Receptor (Brain)",
author = "Moshe Laudon and Nava Zisapel",
note = "Funding Information: This work was supportedb y the Israel Academy of Science and Humanities, Basic Research Foundation.",
year = "1986",
month = mar,
day = "3",
doi = "10.1016/0014-5793(86)80287-3",
language = "אנגלית",
volume = "197",
pages = "9--12",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "1-2",
}