Abstract
The binding of 125I-melatonin, a potent analog of melatonin, to rat brain synaptosomal preparations was investigated. 125I-melatonin bound with high affinity (Kd = 38 nM) to a single class of sites (Bmax = 81 fmol mg protein). Kinetic studies indicated that binding was time-dependent and reversible. Specific 125I-melatonin binding was inhibited by melatonin, and was unaffected by other structurally related compounds including serotonin. Binding of 125I-melatonin was greatly reduced if the synaptosomal preparations were pretreated by heat or trypsin but was unaffected by freeze-thawing. These results suggest that 125I-melatonin may serve as a valuable probe for studying melatonin receptors.
Original language | English |
---|---|
Pages (from-to) | 9-12 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 197 |
Issue number | 1-2 |
DOIs | |
State | Published - 3 Mar 1986 |
Keywords
- Melatonin Receptor (Brain)