Characterization of Ca2+-activated phospholipid-dependent protein kinase C / protein kinase m in guinea pig peritoneal exudate macrophages

Rosa Toper, Edgar Pick

Research output: Contribution to journalArticlepeer-review

Abstract

Protein kinase C (C-kinase) is shown to be present in the cytosolic and particulate fractions of mineral oil induced peritoneal macrophages of guinea pigs. By omission or use of a high concentration of leupeptin, three forms of the enzyme were obtained: 1) stimulant/ Ca2+/phospholipid-dependent C-kinase, eluted from DEAE 52 cellulose at 0.08-0.16 M NaCl; 2) stimulant/Ca2+/phospholipid-independent protein kinase M (M-kinase), and 3) Ca2+-inhibited & stimulant/phospholipid-dependent form of protein kinase, both eluted from DEAE 52 cellulose at 0.18-0.22 M NaCl. Phorbol ester or 1,2-diacylglycerol were used as stimulants. It is suggested that Ca2+-inhibited & stimulant/ phospholipid-dependent protein kinase represents the in vivo form of the M-kinase in intact cells.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume141
Issue number2
DOIs
StatePublished - 15 Dec 1986

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