Characterization of an endopolygalacturonase associated with take-all disease of wheat

S. Dori; J. Hershenhorn; Z. Solel; I. Barash

doi:10.1016/0885-5765(92)90061-Y

Characterization of an endopolygalacturonase associated with take-all disease of wheat

S. Dori^*, J. Hershenhorn, Z. Solel, I. Barash

^*Corresponding author for this work

School of Plant Sciences and Food Security

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A homogeneous endopolygalacturonase preparation was obtained from a culture filtrate of Gaeumannomyces graminis var. tritici by isoelectrofocusing and carboxymethylcellulose chromatography. The enzyme has a molecular weight of 42kDa an isoelectric point at pH 8·1, and a pH optimum at 4·5-5·5. Different fungal isolates secreted similar amounts of endopolygalacturonase. The same enzyme was present in diseased wheat roots. Endopolygalacturonase activity was detected in inoculated wheat roots 2 days prior to appearance of symptoms. Degradation of pectic substances during infection was revealed in root sections stained with ruthenium red. The possible function of the endopolygalacturonase in pathogenesis is discussed.

Original language	English
Pages (from-to)	203-210
Number of pages	8
Journal	Physiological and Molecular Plant Pathology
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/0885-5765(92)90061-Y
State	Published - Mar 1992

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abstract = "A homogeneous endopolygalacturonase preparation was obtained from a culture filtrate of Gaeumannomyces graminis var. tritici by isoelectrofocusing and carboxymethylcellulose chromatography. The enzyme has a molecular weight of 42kDa an isoelectric point at pH 8·1, and a pH optimum at 4·5-5·5. Different fungal isolates secreted similar amounts of endopolygalacturonase. The same enzyme was present in diseased wheat roots. Endopolygalacturonase activity was detected in inoculated wheat roots 2 days prior to appearance of symptoms. Degradation of pectic substances during infection was revealed in root sections stained with ruthenium red. The possible function of the endopolygalacturonase in pathogenesis is discussed.",

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AU - Dori, S.

AU - Hershenhorn, J.

AU - Solel, Z.

AU - Barash, I.

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AB - A homogeneous endopolygalacturonase preparation was obtained from a culture filtrate of Gaeumannomyces graminis var. tritici by isoelectrofocusing and carboxymethylcellulose chromatography. The enzyme has a molecular weight of 42kDa an isoelectric point at pH 8·1, and a pH optimum at 4·5-5·5. Different fungal isolates secreted similar amounts of endopolygalacturonase. The same enzyme was present in diseased wheat roots. Endopolygalacturonase activity was detected in inoculated wheat roots 2 days prior to appearance of symptoms. Degradation of pectic substances during infection was revealed in root sections stained with ruthenium red. The possible function of the endopolygalacturonase in pathogenesis is discussed.

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Characterization of an endopolygalacturonase associated with take-all disease of wheat

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