Characterization of an endopolygalacturonase associated with take-all disease of wheat

S. Dori*, J. Hershenhorn, Z. Solel, I. Barash

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A homogeneous endopolygalacturonase preparation was obtained from a culture filtrate of Gaeumannomyces graminis var. tritici by isoelectrofocusing and carboxymethylcellulose chromatography. The enzyme has a molecular weight of 42kDa an isoelectric point at pH 8·1, and a pH optimum at 4·5-5·5. Different fungal isolates secreted similar amounts of endopolygalacturonase. The same enzyme was present in diseased wheat roots. Endopolygalacturonase activity was detected in inoculated wheat roots 2 days prior to appearance of symptoms. Degradation of pectic substances during infection was revealed in root sections stained with ruthenium red. The possible function of the endopolygalacturonase in pathogenesis is discussed.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalPhysiological and Molecular Plant Pathology
Issue number3
StatePublished - Mar 1992


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