Characterization of a protein, released by the T47D cell line, immunologically related to the major envelope protein of mouse mammary tumor virus

N. Segev, A. Hizi, F. Kirenberg, I. Keydar

Research output: Contribution to journalArticlepeer-review

Abstract

The T47D human mammary adenocarcinoma cell line in vitro releases viral particles as well as soluble proteins. Both fractions were shown to contain antigens that immunologically crossreact with the major glycoprotein (gp52) of mouse mammary tumor virus. The crossreacting antigens are located on polypeptides with apparent molecular weights of about 68,000 and 60,000. The larger one is present in viral particles whereas both are found in the soluble fraction. Both proteins are glycosylated. The human tissue culture proteins differ from gp52 not only in molecular weight but also in charge heterogeneity and in polypeptide profiles obtained after partial proteolysis. The results suggest that there is a restricted similarity between MMTV gp52 and the immunologically related T47D proteins.

Original languageEnglish
Pages (from-to)1531-1535
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number5
DOIs
StatePublished - 1985

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