TY - JOUR
T1 - Characterization and quantitative analysis of serum IgG class and subclass response to Shigella sonnei and Shigella flexneri 2a lipopolysaccharide following natural Shigella infection
AU - Robin, Guy
AU - Cohen, Dani
AU - Orr, Nadav
AU - Markus, Ita
AU - Slepon, Raphael
AU - Ashkenazi, Shai
AU - Keisari, Yona
N1 - Funding Information:
Received 7 August 1996; revised 25 November 1996. Informed consent was obtained from the study participants. The study was approved by the Committee for Research on Human Subjects of the Medical Corps, Israel Defence Force. Financial support: US Army Medical Research and Materiel Command, Fort Detrick, Frederick, Maryland (DAMD17-93-V-3001). This work is a partial fulfillment of the requirements for the Ph.D. degree of G.R. (Department of Human Microbiology, Sackler Faculty of Medicine, Tel Aviv University). Reprints or correspondence: Dr. Guy Robin, Military Post 02149, Israel Defence Force, Israel.
PY - 1997
Y1 - 1997
N2 - The IgG subclass response to Shigella sonnei and Shigella flexneri 2a lipopolysaccharide (LPS) was examined in subjects naturally exposed to these organisms. Affinity-purified LPS antibodies obtained using a column of Shigella LPS bound to epoxy-activated Sepharose 6B were used as standards to calibrate the serum antibody response to natural Shigella infection. The geometric mean concentrations of specific IgG in sera from those not exposed to Shigella organisms were 7.9 μg/mL against S. sonnei LPS and 18.6 μg/mL against S. flexneri 2a LPS. After natural exposure to S. sonnei or S. flexneri 2a, the concentrations rose to 30.3 and 127.9 μg/mL, respectively. IgG2 was the major component in the anti-S. flexneri subclass response, while the anti-S. sonnei response was dominated by IgG1. High levels of IgG1 antibodies before exposure to organisms from either Shigella serogroup correlated with a lower risk of developing symptomatic infection.
AB - The IgG subclass response to Shigella sonnei and Shigella flexneri 2a lipopolysaccharide (LPS) was examined in subjects naturally exposed to these organisms. Affinity-purified LPS antibodies obtained using a column of Shigella LPS bound to epoxy-activated Sepharose 6B were used as standards to calibrate the serum antibody response to natural Shigella infection. The geometric mean concentrations of specific IgG in sera from those not exposed to Shigella organisms were 7.9 μg/mL against S. sonnei LPS and 18.6 μg/mL against S. flexneri 2a LPS. After natural exposure to S. sonnei or S. flexneri 2a, the concentrations rose to 30.3 and 127.9 μg/mL, respectively. IgG2 was the major component in the anti-S. flexneri subclass response, while the anti-S. sonnei response was dominated by IgG1. High levels of IgG1 antibodies before exposure to organisms from either Shigella serogroup correlated with a lower risk of developing symptomatic infection.
UR - http://www.scopus.com/inward/record.url?scp=0030898197&partnerID=8YFLogxK
U2 - 10.1086/516452
DO - 10.1086/516452
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AN - SCOPUS:0030898197
SN - 0022-1899
VL - 175
SP - 1128
EP - 1133
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -