Characteristic attributes limiting the transport rates in NCX orthologs

Bosmat Refaeli, Reuben Hiller, Daniel Khananshvili

Research output: Contribution to journalArticlepeer-review


The Na+/Ca2+ exchangers (NCXs) modulate the Ca2+ signaling and homeostasis in health and disease. The transport cycle turnover rates (kcat) and the kcat/Km values of eukaryotic NCXs are ~104-times higher than those of prokaryotic NCXs. Three ion-coordinating residues (out of twelve) differ between eukaryotic NCXs and NCX_Mj. The replacement of three ion-coordinating residues in NCX_Mj does not increase kcat, probably due to the structural rigidity of NCX_Mj. Phospholipids and cholesterol increase (up to 10-fold) the transport rates in the cardiac NCX1.1, but not in NCX_Mj. A lipid environment can partially contribute to the huge kinetic variances among NCXs.

Original languageEnglish
Article number183792
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number1
StatePublished - 1 Feb 2022


  • Ion-coordinating residues
  • NCX
  • Sodium‑calcium exchanger
  • Transport rates


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