@article{abc94da0ac704de883c6f99968154db4,
title = "Changes to the dynamic nature of hemagglutinin and the emergence of the 2009 pandemic H1N1 influenza virus",
abstract = "The virologic factors that limit the transmission of swine influenza viruses between humans are unresolved. While it has been shown that acquisition of the neuraminidase (NA) and matrix (M) gene segments from a Eurasian-lineage swine virus was required for airborne transmission of the 2009 pandemic H1N1 virus (H1N1pdm09), we show here that an arginine to lysine change in the hemagglutinin (HA) was also necessary. This change at position 149 was distal to the receptor binding site but affected virus-receptor affinity and HA dynamics, allowing the virus to replicate more efficiently in nasal turbinate epithelium and subsequently transmit between ferrets. Receptor affinity should be considered as a factor limiting swine virus spread in humans.",
author = "Yoon, {Sun Woo} and Noam Chen and Ducatez, {Mariette F.} and Ryan McBride and Subrata Barman and Fabrizio, {Thomas P.} and Webster, {Robert G.} and Turkan Haliloglu and Paulson, {James C.} and Russell, {Charles J.} and Tomer Hertz and Nir Ben-Tal and Webby, {Richard J.}",
note = "Funding Information: We thank the following colleagues from St. Jude Children{\textquoteright}s Research Hospital: J.C. Crumpton, B. Marathe, J. Trushar, and A. Webb for excellent technical assistance, C. Guess for editing the manuscript and S.S. Wang, M. Zanin, and H. Zarket for critically reviewing the manuscript. We thank Y.K. Choi (Chungbuk National University, South Korea) and J.K. Kim (Korea University, South Korea) for informative discussions. This work was supported by the National Institute of Allergy and Infectious Diseases (NIAID), Centers of Excellence for Influenza Research and Surveillance (CEIRS) under Contract No. HHSN266200700005C, Cancer Center core grant CA21765, and by ALSAC. N.B.-T. acknowledges the support of the Ministry of Science and Technology, Israel. T.H. acknowledges T{\"U}BİTAK project no: 110T088. N.B.-T. and T.H. acknowledge NATO{\textquoteright}s Emerging Security Challenges Division in the framework of the Science for Peace and Security Programme (SPS Project G4622). J.P. acknowledges the support of NIAID grant number AI058113. S.-W.Y. acknowledges the KRIBB Initiative program (KGM4691511). All work was conducted prior to the US Governments pause on gain of function research.",
year = "2015",
month = aug,
day = "13",
doi = "10.1038/srep12828",
language = "אנגלית",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Research",
}
