Changes in gene expression pattern following granulocyte colony-stimulating factor administration to normal stem cell sibling donors

Ninette Amariglio, Jasmine Jacob-Hirsch, Avichai Shimoni, Merav Leiba, Gideon Rechavi, Arnon Nagler

Research output: Contribution to journalArticlepeer-review

Abstract

Granulocyte colony-stimulating factor (G-CSF) is widely used for the mobilization of hematopoietic stem cells from normal donors. The mechanism of induced mobilization has recently been elucidated. Treatment with G-CSF is considered safe; however, long-term effects are largely unknown. The aim of this study was, therefore, to determine whether it leads to significant changes in gene expression modifications. Affymetrix Gene Chip array technology was used. Samples were collected before and at various time points (up to 9 months) after mobilization. The expression levels of 122 genes were transiently modified before and after mobilization (p < 0.05). Fifty-three genes belonging to cell growth, proliferation and communication gene ontology categories were upregulated, while 69 genes were downregulated. Conclusion: The administration of G-CSF is associated with transient DNA and gene expression modifications in the lymphocytes of normal mobilized donors. A long-term follow-up of stem cell donors is recommended.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalActa Haematologica
Volume117
Issue number2
DOIs
StatePublished - Feb 2007
Externally publishedYes

Keywords

  • Expression level
  • Gene chip array
  • Granulocyte colony-stimulating factor
  • Normal donors
  • Stem cell transplantation

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