TY - JOUR
T1 - Cementum attachment protein enriches putative cementoblastic populations on root surfaces in vitro
AU - Barkana, I.
AU - Narayanan, A. S.
AU - Grosskop, A.
AU - Savion, N.
AU - Pitaru, S.
PY - 2000
Y1 - 2000
N2 - We tested the capacity of cementum attachment protein (CAP) to recruit putative cementoblastic populations to root surfaces in vitro by determining the phenotypic expression of periodontal ligament cloned cell populations. The clones were derived from cells that attached to either CAP-coated (experimental) or uncoated (control) root slices. Root slices were co-cultured with primary human periodontal ligament cells. Cloned and parent populations were analyzed for their capacity to express alkaline phosphatase (AP), osteopontin, bone sialoprotein (BSP), and CAP and to form mineralized tissue in vitro. The percentage of CAP- and BSP-positive clones was significantly higher in the experimental clones than in the controls. The percentage of cells positive for AP, BSP, and CAP was higher in the experimental clones than in their control counterparts. Mineralized tissue formation was observed only in the cell populations derived from the CAP-coated root slices. These results indicate that CAP is capable of recruiting putative cementoblastic populations on root slices in vitro and therefore might play an important role in cementogenesis during periodontal homeostasis and wound healing.
AB - We tested the capacity of cementum attachment protein (CAP) to recruit putative cementoblastic populations to root surfaces in vitro by determining the phenotypic expression of periodontal ligament cloned cell populations. The clones were derived from cells that attached to either CAP-coated (experimental) or uncoated (control) root slices. Root slices were co-cultured with primary human periodontal ligament cells. Cloned and parent populations were analyzed for their capacity to express alkaline phosphatase (AP), osteopontin, bone sialoprotein (BSP), and CAP and to form mineralized tissue in vitro. The percentage of CAP- and BSP-positive clones was significantly higher in the experimental clones than in the controls. The percentage of cells positive for AP, BSP, and CAP was higher in the experimental clones than in their control counterparts. Mineralized tissue formation was observed only in the cell populations derived from the CAP-coated root slices. These results indicate that CAP is capable of recruiting putative cementoblastic populations on root slices in vitro and therefore might play an important role in cementogenesis during periodontal homeostasis and wound healing.
KW - Alkaline phosphatase
KW - Bone sialoprotein
KW - Cementum
KW - Cementum attachment protein
KW - Mineralized tissue
KW - Osteopontin
KW - Periodontal ligament
UR - http://www.scopus.com/inward/record.url?scp=0034441531&partnerID=8YFLogxK
U2 - 10.1177/00220345000790070901
DO - 10.1177/00220345000790070901
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C2 - 11005732
AN - SCOPUS:0034441531
SN - 0022-0345
VL - 79
SP - 1482
EP - 1488
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 7
ER -