Cellular hypersensitivity to neocarzinostatin in ataxia-telangiectasia skin fibroblasts

Yosef Shiloh*, Einat Tabor, Yechiel Becker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Cellular sensitivity of human skin fibroblast strains from three healthy donors, eight ataxiatelangiectasia (A-T) patients belonging to six sibships, and two A-T heterozygotes to the lethal action of the antitumor antibiotic neocarzinostatin was tested, using colony-forming ability as the criterion for survival. All the A-T strains were significantly more sensitive to killing by neocarzinostatin than were the control strains. The average D0for the A-T strains following neocarzinostatin treatment was 14.6 ng/ml, as compared to 37.9 ng/ml for the normal strains. The two A-T heterozygous strains showed intermediate sensitivity with an average D0 of 26.9 ng/ml. Neocarzinostatin sensitivity of A-T cells could therefore serve as a convenient aid for the laboratory diagnosis of A-T. Since A-T cells are also known to be hypersensitive to ionizing radiation and bleomycin, it would appear that they are primarily hypersensitive to DNA-breaking agents.

Original languageEnglish
Pages (from-to)2247-2249
Number of pages3
JournalCancer Research
Issue number6
StatePublished - 1 Jun 1982
Externally publishedYes


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