Cell tri-culture for cardiac vascularization

Ayelet Lesman, Lior Gepstein, Shulamit Levenberg

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Poor graft survival is a critical obstacle toward production of clinically relevant engineered tissues. Here we utilize a multicellular culturing approach for induction of vascular networks embedded within cardiac tissue constructs. The construct is composed of human cardiomyocytes, endothelial cells (ECs), and embryonic fibroblast cells co-seeded onto highly porous three-dimensional (3D) scaffolds. The resulting vascularized cardiac constructs showed microstructural details characteristic of cardiomyocytes and nascent vessels and exhibited synchronous beating activity in vitro. Upon implantation, stable grafts were formed presenting intense vascularization, with evidence of anastomosis between the pre-formed endothelial capillaries and host neovessels.

Original languageEnglish
Title of host publicationCardiac Tissue Engineering
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages7
ISBN (Print)9781493910465
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cardiomyocytes
  • Co-culture
  • Endothelial cells
  • Scaffold
  • Tissue engineering
  • Vascularization


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