Cell surface labeling of embryonic neural retina cells exposed to low temperature, energy inhibitors, cytochalasin B and colchicin

E. Cohen*, C. Gitler, Y. Ben-Shaul

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Large blebs devoid of receptors for hemocyanin-labeled concanavalin A appear on neural retina cells from 8-day chick embryos exposed to low temperature or to potassium cyanide or 2,4(α)-dinitrophenol at room temperature. Labeling with mixed antibodies against a crude retina membrane preparation and with goat anti-rabbit-hemocyanin conjugate showed the same results. Determination of cell ATP content indicated a drop in ATP concentration after exposure to low temperature or to respiration inhibitors. Disruption of microtubules by colchicin inhibited the formation of 'naked' large blebs, whereas cytochalasin B had no such inhibitory effect.

Original languageEnglish
Pages (from-to)333-342
Number of pages10
JournalCell Differentiation
Volume10
Issue number6
DOIs
StatePublished - Dec 1981

Keywords

  • cytoskeleton
  • embryonic cells
  • energy inhibitors
  • scanning electron microscopy
  • surface labeling

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