TY - JOUR
T1 - Cell-membrane receptor classes delimited through cap formation either with diamide or with membrane mobility agent, A2C
AU - Kosower, Nechamas S.
AU - Faltin, Zehava
AU - Kosower, Edward M.
N1 - Funding Information:
The Israeli Cancer Society and the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel, are thanked for support.
PY - 1981/3/16
Y1 - 1981/3/16
N2 - Receptors on normal human peripheral blood lymphocytes can be divided into two classes by means of the capping response exhibited in the presence of the reagents, diamide or colchicine (microtubule-related) and A2C (microtubule-independent). Diamide and colchicine promote capping of concanavalin A (Con A) receptors. Diamide capping is reversible, while colchicine capping is not reversible under the conditions used. A2C does not promote the capping of Con A receptors. In contrast, diamide and colchicine do not affect the rate at which either anti-immunoglobulin (anti-Ig) or wheat germ agglutinin (WGA) receptors cap, but A2C effectively enhances cap formation for both anti-Ig and WGA receptors. The simplicity of the classification method promises to be of use in the investigation of membrane receptors.
AB - Receptors on normal human peripheral blood lymphocytes can be divided into two classes by means of the capping response exhibited in the presence of the reagents, diamide or colchicine (microtubule-related) and A2C (microtubule-independent). Diamide and colchicine promote capping of concanavalin A (Con A) receptors. Diamide capping is reversible, while colchicine capping is not reversible under the conditions used. A2C does not promote the capping of Con A receptors. In contrast, diamide and colchicine do not affect the rate at which either anti-immunoglobulin (anti-Ig) or wheat germ agglutinin (WGA) receptors cap, but A2C effectively enhances cap formation for both anti-Ig and WGA receptors. The simplicity of the classification method promises to be of use in the investigation of membrane receptors.
UR - http://www.scopus.com/inward/record.url?scp=0019491641&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(81)90244-1
DO - 10.1016/0022-1759(81)90244-1
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AN - SCOPUS:0019491641
SN - 0022-1759
VL - 41
SP - 215
EP - 223
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -