Cell cycle-dependent regulation of Double-Strand Break repair: A role for the CDK

Yael Aylon, Martin Kupiec*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

Abstract

DNA Double-Strand Breaks (DSBs) are dangerous lesions that can lead to genomic instability and to cell death. Eukaryotic cells repair DSBs either by nonhomologous end joining (NHEJ) or by homologous recombination (HR). Recent work has allowed to study the ability of yeast cells to repair a single, chromosomal DSB, at different stages of the cell cycle. Yeast cells repair the broken chromosome during the G1 stage only by NHEJ, whereas HR is the mechanism of choice during the rest of the cell cycle. HR does not require duplicated chromatids or passage through S-phase. Control over the fate of the broken chromosome is exerted by Clb-CDK activity, which is required to carry out the first step of HR, ssDNA resection. Similar results in other organisms suggest that this control is a conserved feature in all eukaryotes.

Original languageEnglish
Pages (from-to)258-260
Number of pages3
JournalCell Cycle
Volume4
Issue number2
DOIs
StatePublished - Feb 2005

Keywords

  • DSB repair
  • Gene conversion
  • NHEJ
  • Saccharomyces cerevisiae
  • Yeast
  • cdc28
  • cdc4
  • cdc7
  • sic1

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