Cell biology and evolution of proton pumps

Nathan Nelson*, Carmen Beltrán, Frantisek Supek, Hannah Nelson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


The vacuolar system of eukaryotic cells contains a large number of organelles that are primarily energized by an H+- ATPase that was named V-ATPase. Several genes encoding subunits of the enzyme were cloned and sequenced. The sequence information revealed the relations between V-ATPases and F-ATPases that evolved from common ancestral genes. Genes encoding subunits of V-ATPase in yeast cells were interrupted to yield mutants that are devoid of the enzyme and are sensitive to pH and calcium concentrations in the medium. The mutants were used for the study of structure, function, molecular biology and biogenesis of the V-ATPase. The function of V-ATPases in energizing the vacuolar system of eukaryotes is discussed.

Original languageEnglish
Pages (from-to)150-158
Number of pages9
JournalCellular Physiology and Biochemistry
Issue number3-4
StatePublished - 1992
Externally publishedYes


  • Cloning
  • Evolution
  • F-ATPase
  • V-ATPase
  • Yeast mutants


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