TY - JOUR
T1 - Cell and nucleus refractive-index mapping by interferometric phase microscopy and rapid confocal fluorescence microscopy
AU - Cohen-Maslaton, Shir
AU - Barnea, Itay
AU - Taieb, Almog
AU - Shaked, Natan T.
N1 - Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/9/1
Y1 - 2020/9/1
N2 - We present a multimodal technique for measuring the integral refractive index and the thickness of biological cells and their organelles by integrating interferometric phase microscopy (IPM) and rapid confocal fluorescence microscopy. First, the actual thickness maps of the cellular compartments are reconstructed using the confocal fluorescent sections, and then the optical path difference (OPD) map of the same cell is reconstructed using IPM. Based on the co-registered data, the integral refractive index maps of the cell and its organelles are calculated. This technique enables rapidly measuring refractive index of live, dynamic cells, where IPM provides quantitative imaging capabilities and confocal fluorescence microscopy provides molecular specificity of the cell organelles. We acquire human colorectal adenocarcinoma cells and show that the integral refractive index values are similar for the whole cell, the cytoplasm and the nucleus on the population level, but significantly different on the single cell level.
AB - We present a multimodal technique for measuring the integral refractive index and the thickness of biological cells and their organelles by integrating interferometric phase microscopy (IPM) and rapid confocal fluorescence microscopy. First, the actual thickness maps of the cellular compartments are reconstructed using the confocal fluorescent sections, and then the optical path difference (OPD) map of the same cell is reconstructed using IPM. Based on the co-registered data, the integral refractive index maps of the cell and its organelles are calculated. This technique enables rapidly measuring refractive index of live, dynamic cells, where IPM provides quantitative imaging capabilities and confocal fluorescence microscopy provides molecular specificity of the cell organelles. We acquire human colorectal adenocarcinoma cells and show that the integral refractive index values are similar for the whole cell, the cytoplasm and the nucleus on the population level, but significantly different on the single cell level.
KW - cell imaging
KW - confocal fluorescence microscopy
KW - holographic microscopy
KW - quantitative phase imaging
UR - http://www.scopus.com/inward/record.url?scp=85087441483&partnerID=8YFLogxK
U2 - 10.1002/jbio.202000117
DO - 10.1002/jbio.202000117
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C2 - 32468735
AN - SCOPUS:85087441483
SN - 1864-063X
VL - 13
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 9
M1 - e202000117
ER -