Ceftazidime and vancomycin levels in the aqueous humour and vitreous following intracameral injection in rabbits eyes

Objective. To determine the penetration of ceftazidime and vancomycin into the vitreous cavity of phakic rabbit eyes following intracameral injection Methods. Eighteen healthy phakic albino rabbit eyes were randomized into one of 9 groups. After an anterior chamber paracentesis, 0.1 ml of ceftazidime 2 mg or 4 mg, vancomycin 1 mg or 2 mg, or normal salir e control was injected intracamerally At 1 or 12 hours, aqueous humor and vitr;ous specimens were obtained and assayed to detect levels of ceftazidime or /ancomycin prior to enucleation. Results. Following intracameral injection of ceftazidime 2 mg or 4 mg, detectable aqueous levels (meg/ml) were obtained 1 iour (1714 and 1218) and 12 hours ( 6.3 and 13.4). Vitreous levels of ceftazidime were not detectable 1 hour for either dose of ceftazidime but vitreous levels (m;g/ml) were present at 12 hours for the 2 and 4 mg doses (30.6 and 46.3). Following intracameral injection of vancomycin Img or 2mg, aqueous levels (meg/ml) wtre 1667 and 2529 at one hour and 8 6 and 4.8 at 12 hours. No detectable vitreous levels of either dose of vancomycin were present 1 or 12 hours following intn.cameral injection. Conclusions. In the nontraumatized, phakic rabbit eye followi ig intracameral injection, vitreous levels of ceftazidime can be obtained, while vitn.ous levels of vancomycin are not present at 1 or 12 hours following injection.