TY - JOUR
T1 - cDNA excision in stable retroviral cDNA transfectants is prevented by R removal
AU - Rosin-Arbesfeld, Rina
AU - Gazit, Arnona
AU - Yaniv, Abraham
N1 - Funding Information:
This research was supported by a Grant from the German–Israeli Foundation for Scientific Research and Development (G.I.F.).
PY - 1998/9/29
Y1 - 1998/9/29
N2 - The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.
AB - The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.
UR - http://www.scopus.com/inward/record.url?scp=0032578606&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9381
DO - 10.1006/bbrc.1998.9381
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AN - SCOPUS:0032578606
VL - 250
SP - 747
EP - 750
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -