cDNA excision in stable retroviral cDNA transfectants is prevented by R removal

Research output: Contribution to journalArticlepeer-review

Abstract

The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.

Original languageEnglish
Pages (from-to)747-750
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume250
Issue number3
DOIs
StatePublished - 29 Sep 1998

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