CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow: Role of β1 (CD29)-, β2 (CD18)-related integrins and non-integrin receptors

Boris Shenkman, Grigory Brill, Alexey Solpov, Yuri Vitkovsky, Boris Kuznik, Alexander Koltakov, Shlomo Kotev-Emeth, Naphtali Savion, Ilan Bank

Research output: Contribution to journalArticlepeer-review

Abstract

The role of platelets in T-lymphocytes adhesion is not clear yet. Herpesvirus saimiri (HVS)-infected CD4+ T-lymphocytes were placed into polystyrene plates pre-coated with fibronectin. The adherent T-cells were enumerated by image analysis. Under static condition, 38 ± 10 cells/mm2 adhered and addition of gel-filtered platelets (GFP) and PMA enhanced cell adhesion 4.3- and 4.1-fold. Using PMA plus GFP 11.9-fold enhancement in cell adhesion was achieved. In contrast, under flow (200 s-1), neither basal adhesion nor following separate addition of PMA or GFP was observed, whereas combined addition of PMA and GFP induced noticeable adhesion (34 cells/mm2). The adhesion was inhibited by blockade of α5-integrin (CD49e, 87%), β2-integrin (CD18, 78%), CD40L (60%), PSGL-1 (CD162, 60%), and CD40L plus PSGL-1 (83%). Thus, activated platelets promote activated T-cell adhesion to fibronectin under flow via integrins (α5β1, and αLβ2), CD40-CD40L and P-selectin-PSGL-1 mediated interactions.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalCellular Immunology
Volume242
Issue number1
DOIs
StatePublished - Jul 2006

Keywords

  • Adhesion
  • CD4 T-lymphocytes
  • Cone and plate(let) analyzer
  • Fibronectin
  • Platelets
  • Shear stress

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