TY - JOUR
T1 - CD4+ lymphocytes require platelets for adhesion to immobilized fibronectin in flow
T2 - Role of β1 (CD29)-, β2 (CD18)-related integrins and non-integrin receptors
AU - Shenkman, Boris
AU - Brill, Grigory
AU - Solpov, Alexey
AU - Vitkovsky, Yuri
AU - Kuznik, Boris
AU - Koltakov, Alexander
AU - Kotev-Emeth, Shlomo
AU - Savion, Naphtali
AU - Bank, Ilan
PY - 2006/7
Y1 - 2006/7
N2 - The role of platelets in T-lymphocytes adhesion is not clear yet. Herpesvirus saimiri (HVS)-infected CD4+ T-lymphocytes were placed into polystyrene plates pre-coated with fibronectin. The adherent T-cells were enumerated by image analysis. Under static condition, 38 ± 10 cells/mm2 adhered and addition of gel-filtered platelets (GFP) and PMA enhanced cell adhesion 4.3- and 4.1-fold. Using PMA plus GFP 11.9-fold enhancement in cell adhesion was achieved. In contrast, under flow (200 s-1), neither basal adhesion nor following separate addition of PMA or GFP was observed, whereas combined addition of PMA and GFP induced noticeable adhesion (34 cells/mm2). The adhesion was inhibited by blockade of α5-integrin (CD49e, 87%), β2-integrin (CD18, 78%), CD40L (60%), PSGL-1 (CD162, 60%), and CD40L plus PSGL-1 (83%). Thus, activated platelets promote activated T-cell adhesion to fibronectin under flow via integrins (α5β1, and αLβ2), CD40-CD40L and P-selectin-PSGL-1 mediated interactions.
AB - The role of platelets in T-lymphocytes adhesion is not clear yet. Herpesvirus saimiri (HVS)-infected CD4+ T-lymphocytes were placed into polystyrene plates pre-coated with fibronectin. The adherent T-cells were enumerated by image analysis. Under static condition, 38 ± 10 cells/mm2 adhered and addition of gel-filtered platelets (GFP) and PMA enhanced cell adhesion 4.3- and 4.1-fold. Using PMA plus GFP 11.9-fold enhancement in cell adhesion was achieved. In contrast, under flow (200 s-1), neither basal adhesion nor following separate addition of PMA or GFP was observed, whereas combined addition of PMA and GFP induced noticeable adhesion (34 cells/mm2). The adhesion was inhibited by blockade of α5-integrin (CD49e, 87%), β2-integrin (CD18, 78%), CD40L (60%), PSGL-1 (CD162, 60%), and CD40L plus PSGL-1 (83%). Thus, activated platelets promote activated T-cell adhesion to fibronectin under flow via integrins (α5β1, and αLβ2), CD40-CD40L and P-selectin-PSGL-1 mediated interactions.
KW - Adhesion
KW - CD4 T-lymphocytes
KW - Cone and plate(let) analyzer
KW - Fibronectin
KW - Platelets
KW - Shear stress
UR - http://www.scopus.com/inward/record.url?scp=33845412246&partnerID=8YFLogxK
U2 - 10.1016/j.cellimm.2006.09.005
DO - 10.1016/j.cellimm.2006.09.005
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AN - SCOPUS:33845412246
SN - 0008-8749
VL - 242
SP - 52
EP - 59
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -