CD4+ lymphocytes as a primary cellular target for BAT mAb stimulation

A. Raiter, G. Rodionov, A. Novogrodsky, B. Hardy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

BAT is a monoclonal antibody (mAb) produced against membranes of a human Burkitt lymphoma cell line (Daudi) that was selected for its ability to stimulate lymphocyte proliferation. BAT manifests anti-tumor properties in mice bearing a variety of murine tumors. BAT also induced regression of human tumors inoculated into SCID mice that had been engrafted with human lymphocytes. The anti-tumor activity of BAT was related to its immune stimulatory properties. Previous data indicated that T lymphocytes and NK cells mediate in vivo the anti-tumor activity. In order to define the primary target cell for BAT stimulatory activity, the in vitro stimulatory effect of BAT on purified lymphocyte subpopulations was investigated. Human CD4+, CD8+ T cells and CD56+ NK cells were purified and their in vitro response to BAT was investigated. Results indicate that BAT selectively stimulated CD4+ cells as assessed by proliferation and secretion of IFN-y. FACS analysis has also revealed a selective increase in BAT antigen on CD4+ T cells that were cultured with BAT antibody. The effector cells that mediate BAT-induced tumor eradication may, however, be distinct from those that serve as the primary cellular target of the antibody. Cytokines such as IFN-γ that are produced by CD4+ cells may be involved in activation of additional cell types that may be involved in tumor destruction.

Original languageEnglish
Pages (from-to)1623-1628
Number of pages6
JournalInternational Immunology
Volume12
Issue number11
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Agonistic antibody
  • BAT receptors
  • IFN-γ
  • Immune stimulation
  • T cell activation

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