Caveolin-1 controls BRCA1 gene expression and cellular localization in human breast cancer cells

Chen Glait; Dana Ravid; Sam W. Lee; Mordechai Liscovitch; Haim Werner

doi:10.1016/j.febslet.2006.08.071

Caveolin-1 controls BRCA1 gene expression and cellular localization in human breast cancer cells

Chen Glait, Dana Ravid, Sam W. Lee, Mordechai Liscovitch, Haim Werner^*

^*Corresponding author for this work

Human Molecular Genetics Biochemistry

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The role of caveolae and the caveolin proteins in cancer has been the subject of extensive research. BRCA1 participates in multiple biological pathways including DNA damage repair, transcriptional control, cell growth, apoptosis, and others. Little information, however, is available regarding the cellular mechanisms that control BRCA1 gene expression. The present study examined the potential regulation of BRCA1 gene expression and subcellular localization by Cav-1. Results of Western blots, RT-PCR, and transfection experiments showed that Cav-1 enhances BRCA1 protein and mRNA levels via a mechanism that involves transactivation of the BRCA1 promoter and which is p53-dependent. In addition, immunostaining experiments demonstrate that Cav-1 induced the cytoplasmic sequestration of BRCA1.

Original language	English
Pages (from-to)	5268-5274
Number of pages	7
Journal	FEBS Letters
Volume	580
Issue number	22
DOIs	https://doi.org/10.1016/j.febslet.2006.08.071
State	Published - 2 Oct 2006

Keywords

BRCA1
Breast cancer
Caveolae
Caveolin-1
Gene expression

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.febslet.2006.08.071

Cite this

@article{1de2e64430bf4c9f993c2bc338edfc79,

title = "Caveolin-1 controls BRCA1 gene expression and cellular localization in human breast cancer cells",

abstract = "The role of caveolae and the caveolin proteins in cancer has been the subject of extensive research. BRCA1 participates in multiple biological pathways including DNA damage repair, transcriptional control, cell growth, apoptosis, and others. Little information, however, is available regarding the cellular mechanisms that control BRCA1 gene expression. The present study examined the potential regulation of BRCA1 gene expression and subcellular localization by Cav-1. Results of Western blots, RT-PCR, and transfection experiments showed that Cav-1 enhances BRCA1 protein and mRNA levels via a mechanism that involves transactivation of the BRCA1 promoter and which is p53-dependent. In addition, immunostaining experiments demonstrate that Cav-1 induced the cytoplasmic sequestration of BRCA1.",

keywords = "BRCA1, Breast cancer, Caveolae, Caveolin-1, Gene expression",

author = "Chen Glait and Dana Ravid and Lee, {Sam W.} and Mordechai Liscovitch and Haim Werner",

year = "2006",

month = oct,

day = "2",

doi = "10.1016/j.febslet.2006.08.071",

language = "אנגלית",

volume = "580",

pages = "5268--5274",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "22",

}

TY - JOUR

T1 - Caveolin-1 controls BRCA1 gene expression and cellular localization in human breast cancer cells

AU - Glait, Chen

AU - Ravid, Dana

AU - Lee, Sam W.

AU - Liscovitch, Mordechai

AU - Werner, Haim

PY - 2006/10/2

Y1 - 2006/10/2

N2 - The role of caveolae and the caveolin proteins in cancer has been the subject of extensive research. BRCA1 participates in multiple biological pathways including DNA damage repair, transcriptional control, cell growth, apoptosis, and others. Little information, however, is available regarding the cellular mechanisms that control BRCA1 gene expression. The present study examined the potential regulation of BRCA1 gene expression and subcellular localization by Cav-1. Results of Western blots, RT-PCR, and transfection experiments showed that Cav-1 enhances BRCA1 protein and mRNA levels via a mechanism that involves transactivation of the BRCA1 promoter and which is p53-dependent. In addition, immunostaining experiments demonstrate that Cav-1 induced the cytoplasmic sequestration of BRCA1.

AB - The role of caveolae and the caveolin proteins in cancer has been the subject of extensive research. BRCA1 participates in multiple biological pathways including DNA damage repair, transcriptional control, cell growth, apoptosis, and others. Little information, however, is available regarding the cellular mechanisms that control BRCA1 gene expression. The present study examined the potential regulation of BRCA1 gene expression and subcellular localization by Cav-1. Results of Western blots, RT-PCR, and transfection experiments showed that Cav-1 enhances BRCA1 protein and mRNA levels via a mechanism that involves transactivation of the BRCA1 promoter and which is p53-dependent. In addition, immunostaining experiments demonstrate that Cav-1 induced the cytoplasmic sequestration of BRCA1.

KW - BRCA1

KW - Breast cancer

KW - Caveolae

KW - Caveolin-1

KW - Gene expression

UR - http://www.scopus.com/inward/record.url?scp=33748765820&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.08.071

DO - 10.1016/j.febslet.2006.08.071

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:33748765820

SN - 0014-5793

VL - 580

SP - 5268

EP - 5274

JO - FEBS Letters

JF - FEBS Letters

IS - 22

ER -

Caveolin-1 controls BRCA1 gene expression and cellular localization in human breast cancer cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this