Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California

Kuniyoshi Kanai; Meredith Whiteside; Michael Wong; Tammy La; Maryam Nassiri; Sam Lee; Sze Kei Yeung; Adrienne Coulter; Mareena Roufail; Kevin Ruder; Cindi Chen; David Liu; Thomas Abraham; Armin Hinterwirth; Thomas M. Lietman; Thuy Doan; Gerami D. Seitzman; Lalitha Prajna; N. Venkatesh Prajna; Ramesh Gunasekaran; Sankalp Singh Sharma; Vishnu Teja; Meenu Chaudhary; Sanjeeta Sitaula; Ali Sié; Boubacar Coulibaly; Mamadou Bountogo; Thanapong Somkijrungroj; Vannarut Satitpitakul; Huy Tran; Linh Hoàng Mai; Tho H. Xuân; Yen Tran; Cristhian A. Urzua; Fabian Vega; Felipe Salgado; Loreto Cuitino; Fernando Pérez Pérez; Jaime MacÍas Martínez; Van Charles Lansingh; Sukhumal Thanapaisal; Wipada Laovirojjanakul; George McKie; Kenia Chavez; Travis Redd; Winston Chamberlain; Angel Cheng; Vivien Tham; Wiwan Sansanayudh; Abba Kaka Hajia Yakoura; Abdou Amza; Abdoul Salam Youssoufou Souley; Adam Nouhou Diori; Beido Nassirou; Boubacar Kadri; Boubacar Mariama; Cissé Mamadou Ibrahim; Lamyne Aboubacar Roufaye; Ramatou Boulhassane; Saley Ali; Zakou Abdou; Lee Goren; Ruti Sella; Clare Kelliher; Laura Green; Hon Shing Ong; Jod Mehta; Yu Chi Liu; Benjamin A. Pinsky; De Kuang Hwang; Nai Wen Fan; Hong Sheng Chiong; Javier Lacorzana; Maria Cabrera-Aguas; Stephanie Watson; Edmund Tsui; Joana Ramirez; Nina M. Cherian; Rachel Feit-Leichman; Reginald E. Hughes; Tania Onclinx; Carol Yu; Esmeralda McClean; Iliana Molina; Armin Hinterwirth; Cindi Chen; Danny Yu; David Liu; Elodie Lebas; Emily Colby; Gerami Seitzman; Kevin Ruder; Lina Zhong; Michael Deiner; Thomas Abraham; Thomas Lietman; Thuy Doan; Travis Porco; Stephen McLeod; Kuniyoshi Kanai; Meredith Whiteside; Steven Yeh; Tolulope Fashina; James Chodosh; Bridgit Tarkap; Jambi N. Garap; Magdalene Mangot; Edwin Amel; Fasihah Taleo; Johnson Kasso; Kalbule Willie; Madopule Nanu; Prudence Rymill; Anthony W. Solomon

doi:10.1097/OPX.0000000000002010

Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California

Kuniyoshi Kanai^*, Meredith Whiteside, Michael Wong, Tammy La, Maryam Nassiri, Sam Lee, Sze Kei Yeung, Adrienne Coulter, Mareena Roufail, Kevin Ruder, Cindi Chen, David Liu, Thomas Abraham, Armin Hinterwirth, Thomas M. Lietman, Thuy Doan, Gerami D. Seitzman, Lalitha Prajna, N. Venkatesh Prajna, Ramesh GunasekaranSankalp Singh Sharma, Vishnu Teja, Meenu Chaudhary, Sanjeeta Sitaula, Ali Sié, Boubacar Coulibaly, Mamadou Bountogo, Thanapong Somkijrungroj, Vannarut Satitpitakul, Huy Tran, Linh Hoàng Mai, Tho H. Xuân, Yen Tran, Cristhian A. Urzua, Fabian Vega, Felipe Salgado, Loreto Cuitino, Fernando Pérez Pérez, Jaime MacÍas Martínez, Van Charles Lansingh, Sukhumal Thanapaisal, Wipada Laovirojjanakul, George McKie, Kenia Chavez, Travis Redd, Winston Chamberlain, Angel Cheng, Vivien Tham, Wiwan Sansanayudh, Abba Kaka Hajia Yakoura, Abdou Amza, Abdoul Salam Youssoufou Souley, Adam Nouhou Diori, Beido Nassirou, Boubacar Kadri, Boubacar Mariama, Cissé Mamadou Ibrahim, Lamyne Aboubacar Roufaye, Ramatou Boulhassane, Saley Ali, Zakou Abdou, Lee Goren, Ruti Sella, Clare Kelliher, Laura Green, Hon Shing Ong, Jod Mehta, Yu Chi Liu, Benjamin A. Pinsky, De Kuang Hwang, Nai Wen Fan, Hong Sheng Chiong, Javier Lacorzana, Maria Cabrera-Aguas, Stephanie Watson, Edmund Tsui, Joana Ramirez, Nina M. Cherian, Rachel Feit-Leichman, Reginald E. Hughes, Tania Onclinx, Carol Yu, Esmeralda McClean, Iliana Molina, Armin Hinterwirth, Cindi Chen, Danny Yu, David Liu, Elodie Lebas, Emily Colby, Gerami Seitzman, Kevin Ruder, Lina Zhong, Michael Deiner, Thomas Abraham, Thomas Lietman, Thuy Doan, Travis Porco, Stephen McLeod, Kuniyoshi Kanai^*, Meredith Whiteside, Steven Yeh, Tolulope Fashina, James Chodosh, Bridgit Tarkap, Jambi N. Garap, Magdalene Mangot, Edwin Amel, Fasihah Taleo, Johnson Kasso, Kalbule Willie, Madopule Nanu, Prudence Rymill, Anthony W. Solomon

^*Corresponding author for this work

Ophthalmology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

SIGNIFICANCE Acute infectious conjunctivitis poses significant challenges to eye care providers. It can be highly transmissible, and because etiology is often presumed, correct treatment and management can be difficult. This study uses unbiased deep sequencing to identify causative pathogens of infectious conjunctivitis, potentially allowing for improved approaches to diagnosis and management. PURPOSES This study aimed to identify associated pathogens of acute infectious conjunctivitis in a single ambulatory eye care center. CASE REPORTS This study included patients who presented to the University of California Berkeley eye center with signs and symptoms suggestive of infectious conjunctivitis. From December 2021 to July 2021, samples were collected from seven subjects (ages ranging from 18 to 38). Deep sequencing identified associated pathogens in five of seven samples, including human adenovirus D, Haemophilus influenzae, Chlamydia trachomatis, and human coronavirus 229E. CONCLUSIONS Unbiased deep sequencing identified some unexpected pathogens in subjects with acute infectious conjunctivitis. Human adenovirus D was recovered from only one patient in this series. Although all samples were obtained during the COVID-19 pandemic, only one case of human coronavirus 229E and no SARS-CoV-2 were identified.

Original language	English
Pages (from-to)	276-280
Number of pages	5
Journal	Optometry and Vision Science
Volume	100
Issue number	4
DOIs	https://doi.org/10.1097/OPX.0000000000002010
State	Published - 1 Apr 2023

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10.1097/OPX.0000000000002010

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Kanai, K., Whiteside, M., Wong, M., La, T., Nassiri, M., Lee, S., Yeung, S. K., Coulter, A., Roufail, M., Ruder, K., Chen, C., Liu, D., Abraham, T., Hinterwirth, A., Lietman, T. M., Doan, T., Seitzman, G. D., Prajna, L., Prajna, N. V., ... Solomon, A. W. (2023). Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California. Optometry and Vision Science, 100(4), 276-280. https://doi.org/10.1097/OPX.0000000000002010

@article{e40cf5c01a854f7191d4b41bb9e15085,

title = "Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California",

abstract = "SIGNIFICANCE Acute infectious conjunctivitis poses significant challenges to eye care providers. It can be highly transmissible, and because etiology is often presumed, correct treatment and management can be difficult. This study uses unbiased deep sequencing to identify causative pathogens of infectious conjunctivitis, potentially allowing for improved approaches to diagnosis and management. PURPOSES This study aimed to identify associated pathogens of acute infectious conjunctivitis in a single ambulatory eye care center. CASE REPORTS This study included patients who presented to the University of California Berkeley eye center with signs and symptoms suggestive of infectious conjunctivitis. From December 2021 to July 2021, samples were collected from seven subjects (ages ranging from 18 to 38). Deep sequencing identified associated pathogens in five of seven samples, including human adenovirus D, Haemophilus influenzae, Chlamydia trachomatis, and human coronavirus 229E. CONCLUSIONS Unbiased deep sequencing identified some unexpected pathogens in subjects with acute infectious conjunctivitis. Human adenovirus D was recovered from only one patient in this series. Although all samples were obtained during the COVID-19 pandemic, only one case of human coronavirus 229E and no SARS-CoV-2 were identified.",

author = "Kuniyoshi Kanai and Meredith Whiteside and Michael Wong and Tammy La and Maryam Nassiri and Sam Lee and Yeung, {Sze Kei} and Adrienne Coulter and Mareena Roufail and Kevin Ruder and Cindi Chen and David Liu and Thomas Abraham and Armin Hinterwirth and Lietman, {Thomas M.} and Thuy Doan and Seitzman, {Gerami D.} and Lalitha Prajna and Prajna, {N. Venkatesh} and Ramesh Gunasekaran and Sharma, {Sankalp Singh} and Vishnu Teja and Meenu Chaudhary and Sanjeeta Sitaula and Ali Si{\'e} and Boubacar Coulibaly and Mamadou Bountogo and Thanapong Somkijrungroj and Vannarut Satitpitakul and Huy Tran and Mai, {Linh Ho{\`a}ng} and Xu{\^a}n, {Tho H.} and Yen Tran and Urzua, {Cristhian A.} and Fabian Vega and Felipe Salgado and Loreto Cuitino and P{\'e}rez, {Fernando P{\'e}rez} and Mart{\'i}nez, {Jaime Mac{\'I}as} and Lansingh, {Van Charles} and Sukhumal Thanapaisal and Wipada Laovirojjanakul and George McKie and Kenia Chavez and Travis Redd and Winston Chamberlain and Angel Cheng and Vivien Tham and Wiwan Sansanayudh and Yakoura, {Abba Kaka Hajia} and Abdou Amza and Souley, {Abdoul Salam Youssoufou} and Diori, {Adam Nouhou} and Beido Nassirou and Boubacar Kadri and Boubacar Mariama and Ibrahim, {Ciss{\'e} Mamadou} and Roufaye, {Lamyne Aboubacar} and Ramatou Boulhassane and Saley Ali and Zakou Abdou and Lee Goren and Ruti Sella and Clare Kelliher and Laura Green and Ong, {Hon Shing} and Jod Mehta and Liu, {Yu Chi} and Pinsky, {Benjamin A.} and Hwang, {De Kuang} and Fan, {Nai Wen} and Chiong, {Hong Sheng} and Javier Lacorzana and Maria Cabrera-Aguas and Stephanie Watson and Edmund Tsui and Joana Ramirez and Cherian, {Nina M.} and Rachel Feit-Leichman and Hughes, {Reginald E.} and Tania Onclinx and Carol Yu and Esmeralda McClean and Iliana Molina and Armin Hinterwirth and Cindi Chen and Danny Yu and David Liu and Elodie Lebas and Emily Colby and Gerami Seitzman and Kevin Ruder and Lina Zhong and Michael Deiner and Thomas Abraham and Thomas Lietman and Thuy Doan and Travis Porco and Stephen McLeod and Kuniyoshi Kanai and Meredith Whiteside and Steven Yeh and Tolulope Fashina and James Chodosh and Bridgit Tarkap and Garap, {Jambi N.} and Magdalene Mangot and Edwin Amel and Fasihah Taleo and Johnson Kasso and Kalbule Willie and Madopule Nanu and Prudence Rymill and Solomon, {Anthony W.}",

note = "Publisher Copyright: {\textcopyright} Lippincott Williams & Wilkins.",

year = "2023",

month = apr,

day = "1",

doi = "10.1097/OPX.0000000000002010",

language = "אנגלית",

volume = "100",

pages = "276--280",

journal = "Optometry and Vision Science",

issn = "1040-5488",

publisher = "Lippincott Williams and Wilkins Ltd.",

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Kanai, K, Whiteside, M, Wong, M, La, T, Nassiri, M, Lee, S, Yeung, SK, Coulter, A, Roufail, M, Ruder, K, Chen, C, Liu, D, Abraham, T, Hinterwirth, A, Lietman, TM, Doan, T, Seitzman, GD, Prajna, L, Prajna, NV, Gunasekaran, R, Sharma, SS, Teja, V, Chaudhary, M, Sitaula, S, Sié, A, Coulibaly, B, Bountogo, M, Somkijrungroj, T, Satitpitakul, V, Tran, H, Mai, LH, Xuân, TH, Tran, Y, Urzua, CA, Vega, F, Salgado, F, Cuitino, L, Pérez, FP, Martínez, JM, Lansingh, VC, Thanapaisal, S, Laovirojjanakul, W, McKie, G, Chavez, K, Redd, T, Chamberlain, W, Cheng, A, Tham, V, Sansanayudh, W, Yakoura, AKH, Amza, A, Souley, ASY, Diori, AN, Nassirou, B, Kadri, B, Mariama, B, Ibrahim, CM, Roufaye, LA, Boulhassane, R, Ali, S, Abdou, Z, Goren, L, Sella, R, Kelliher, C, Green, L, Ong, HS, Mehta, J, Liu, YC, Pinsky, BA, Hwang, DK, Fan, NW, Chiong, HS, Lacorzana, J, Cabrera-Aguas, M, Watson, S, Tsui, E, Ramirez, J, Cherian, NM, Feit-Leichman, R, Hughes, RE, Onclinx, T, Yu, C, McClean, E, Molina, I, Hinterwirth, A, Chen, C, Yu, D, Liu, D, Lebas, E, Colby, E, Seitzman, G, Ruder, K, Zhong, L, Deiner, M, Abraham, T, Lietman, T, Doan, T, Porco, T, McLeod, S, Kanai, K, Whiteside, M, Yeh, S, Fashina, T, Chodosh, J, Tarkap, B, Garap, JN, Mangot, M, Amel, E, Taleo, F, Kasso, J, Willie, K, Nanu, M, Rymill, P & Solomon, AW 2023, 'Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California', Optometry and Vision Science, vol. 100, no. 4, pp. 276-280. https://doi.org/10.1097/OPX.0000000000002010

TY - JOUR

T1 - Case Series

T2 - Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California

AU - Kanai, Kuniyoshi

AU - Whiteside, Meredith

AU - Wong, Michael

AU - La, Tammy

AU - Nassiri, Maryam

AU - Lee, Sam

AU - Yeung, Sze Kei

AU - Coulter, Adrienne

AU - Roufail, Mareena

AU - Ruder, Kevin

AU - Chen, Cindi

AU - Liu, David

AU - Abraham, Thomas

AU - Hinterwirth, Armin

AU - Lietman, Thomas M.

AU - Doan, Thuy

AU - Seitzman, Gerami D.

AU - Prajna, Lalitha

AU - Prajna, N. Venkatesh

AU - Gunasekaran, Ramesh

AU - Sharma, Sankalp Singh

AU - Teja, Vishnu

AU - Chaudhary, Meenu

AU - Sitaula, Sanjeeta

AU - Sié, Ali

AU - Coulibaly, Boubacar

AU - Bountogo, Mamadou

AU - Somkijrungroj, Thanapong

AU - Satitpitakul, Vannarut

AU - Tran, Huy

AU - Mai, Linh Hoàng

AU - Xuân, Tho H.

AU - Tran, Yen

AU - Urzua, Cristhian A.

AU - Vega, Fabian

AU - Salgado, Felipe

AU - Cuitino, Loreto

AU - Pérez, Fernando Pérez

AU - Martínez, Jaime MacÍas

AU - Lansingh, Van Charles

AU - Thanapaisal, Sukhumal

AU - Laovirojjanakul, Wipada

AU - McKie, George

AU - Chavez, Kenia

AU - Redd, Travis

AU - Chamberlain, Winston

AU - Cheng, Angel

AU - Tham, Vivien

AU - Sansanayudh, Wiwan

AU - Yakoura, Abba Kaka Hajia

AU - Amza, Abdou

AU - Souley, Abdoul Salam Youssoufou

AU - Diori, Adam Nouhou

AU - Nassirou, Beido

AU - Kadri, Boubacar

AU - Mariama, Boubacar

AU - Ibrahim, Cissé Mamadou

AU - Roufaye, Lamyne Aboubacar

AU - Boulhassane, Ramatou

AU - Ali, Saley

AU - Abdou, Zakou

AU - Goren, Lee

AU - Sella, Ruti

AU - Kelliher, Clare

AU - Green, Laura

AU - Ong, Hon Shing

AU - Mehta, Jod

AU - Liu, Yu Chi

AU - Pinsky, Benjamin A.

AU - Hwang, De Kuang

AU - Fan, Nai Wen

AU - Chiong, Hong Sheng

AU - Lacorzana, Javier

AU - Cabrera-Aguas, Maria

AU - Watson, Stephanie

AU - Tsui, Edmund

AU - Ramirez, Joana

AU - Cherian, Nina M.

AU - Feit-Leichman, Rachel

AU - Hughes, Reginald E.

AU - Onclinx, Tania

AU - Yu, Carol

AU - McClean, Esmeralda

AU - Molina, Iliana

AU - Hinterwirth, Armin

AU - Chen, Cindi

AU - Yu, Danny

AU - Liu, David

AU - Lebas, Elodie

AU - Colby, Emily

AU - Seitzman, Gerami

AU - Ruder, Kevin

AU - Zhong, Lina

AU - Deiner, Michael

AU - Abraham, Thomas

AU - Lietman, Thomas

AU - Doan, Thuy

AU - Porco, Travis

AU - McLeod, Stephen

AU - Kanai, Kuniyoshi

AU - Whiteside, Meredith

AU - Yeh, Steven

AU - Fashina, Tolulope

AU - Chodosh, James

AU - Tarkap, Bridgit

AU - Garap, Jambi N.

AU - Mangot, Magdalene

AU - Amel, Edwin

AU - Taleo, Fasihah

AU - Kasso, Johnson

AU - Willie, Kalbule

AU - Nanu, Madopule

AU - Rymill, Prudence

AU - Solomon, Anthony W.

PY - 2023/4/1

Y1 - 2023/4/1

N2 - SIGNIFICANCE Acute infectious conjunctivitis poses significant challenges to eye care providers. It can be highly transmissible, and because etiology is often presumed, correct treatment and management can be difficult. This study uses unbiased deep sequencing to identify causative pathogens of infectious conjunctivitis, potentially allowing for improved approaches to diagnosis and management. PURPOSES This study aimed to identify associated pathogens of acute infectious conjunctivitis in a single ambulatory eye care center. CASE REPORTS This study included patients who presented to the University of California Berkeley eye center with signs and symptoms suggestive of infectious conjunctivitis. From December 2021 to July 2021, samples were collected from seven subjects (ages ranging from 18 to 38). Deep sequencing identified associated pathogens in five of seven samples, including human adenovirus D, Haemophilus influenzae, Chlamydia trachomatis, and human coronavirus 229E. CONCLUSIONS Unbiased deep sequencing identified some unexpected pathogens in subjects with acute infectious conjunctivitis. Human adenovirus D was recovered from only one patient in this series. Although all samples were obtained during the COVID-19 pandemic, only one case of human coronavirus 229E and no SARS-CoV-2 were identified.

AB - SIGNIFICANCE Acute infectious conjunctivitis poses significant challenges to eye care providers. It can be highly transmissible, and because etiology is often presumed, correct treatment and management can be difficult. This study uses unbiased deep sequencing to identify causative pathogens of infectious conjunctivitis, potentially allowing for improved approaches to diagnosis and management. PURPOSES This study aimed to identify associated pathogens of acute infectious conjunctivitis in a single ambulatory eye care center. CASE REPORTS This study included patients who presented to the University of California Berkeley eye center with signs and symptoms suggestive of infectious conjunctivitis. From December 2021 to July 2021, samples were collected from seven subjects (ages ranging from 18 to 38). Deep sequencing identified associated pathogens in five of seven samples, including human adenovirus D, Haemophilus influenzae, Chlamydia trachomatis, and human coronavirus 229E. CONCLUSIONS Unbiased deep sequencing identified some unexpected pathogens in subjects with acute infectious conjunctivitis. Human adenovirus D was recovered from only one patient in this series. Although all samples were obtained during the COVID-19 pandemic, only one case of human coronavirus 229E and no SARS-CoV-2 were identified.

UR - http://www.scopus.com/inward/record.url?scp=85153900295&partnerID=8YFLogxK

U2 - 10.1097/OPX.0000000000002010

DO - 10.1097/OPX.0000000000002010

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C2 - 36880993

AN - SCOPUS:85153900295

SN - 1040-5488

VL - 100

SP - 276

EP - 280

JO - Optometry and Vision Science

JF - Optometry and Vision Science

IS - 4

ER -

Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California

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