TY - JOUR
T1 - Cascade Mesophase Transitions of Multi-enzyme Responsive Polymeric Formulations
AU - Rathee, Parul
AU - Edelstein-Pardo, Nicole
AU - Koren, Gil
AU - Beck, Roy
AU - Amir, Roey J.
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society
PY - 2024/6/10
Y1 - 2024/6/10
N2 - Studying how synthetic polymer assemblies respond to sequential enzymatic stimuli can uncover intricate interactions in biological systems. Using amidase- and esterase-responsive PEG-based diblock (DBA) and triblock amphiphiles (TBAs), we created two distinct formulations: amidase-responsive DBA with esterase-responsive TBA and vice versa. We studied their cascade responses to the two enzymes and the sequence of their introduction. These formulations underwent cascade mesophase transitions upon the addition of the DBA-degrading enzyme, transitioning from (i) coassembled micelles to (ii) triblock-based hydrogel, and ultimately to (iii) dissolved polymers when exposed to the TBA hydrolyzing enzyme. The specific pathway of the two mesophase transitions depended on the compositions of the formulations and the enzyme introduction sequence. The results highlight the potential for designing polymeric formulations with programmable multistep enzymatic responses, mimicking the complex behavior of biological macromolecules.
AB - Studying how synthetic polymer assemblies respond to sequential enzymatic stimuli can uncover intricate interactions in biological systems. Using amidase- and esterase-responsive PEG-based diblock (DBA) and triblock amphiphiles (TBAs), we created two distinct formulations: amidase-responsive DBA with esterase-responsive TBA and vice versa. We studied their cascade responses to the two enzymes and the sequence of their introduction. These formulations underwent cascade mesophase transitions upon the addition of the DBA-degrading enzyme, transitioning from (i) coassembled micelles to (ii) triblock-based hydrogel, and ultimately to (iii) dissolved polymers when exposed to the TBA hydrolyzing enzyme. The specific pathway of the two mesophase transitions depended on the compositions of the formulations and the enzyme introduction sequence. The results highlight the potential for designing polymeric formulations with programmable multistep enzymatic responses, mimicking the complex behavior of biological macromolecules.
UR - http://www.scopus.com/inward/record.url?scp=85193779908&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.4c00221
DO - 10.1021/acs.biomac.4c00221
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 38776179
AN - SCOPUS:85193779908
SN - 1525-7797
VL - 25
SP - 3607
EP - 3619
JO - Biomacromolecules
JF - Biomacromolecules
IS - 6
ER -