Cardiomyocytes in culture - A model to study the cellular actions of amiodarone

G. Kessler-Icekson*, H. Schlesinger, M. Dialdetti, C. Brodie, S. R. Sampson, M. Bergman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The extensive use of amiodarone as an anti-arrhythmic drug is hampered by numerous side effects and by insufficient knowledge of its cellular action. The use of cell cultures for studying the mechanism of amiodarone action has been questioned, since available information has indicated that the doses employed for the experiments induce cell damage. We have defined conditions to obtain the amiodarone effect on cardiac cells in culture with no detectable damage. Amiodarone, 1 μg/ml, a concentration comparable to serum levels of the drug in acute and chronically treated humans and rats, reduces cell contractions, modifies membrane electrical properties accordingly, increases ATP content, but does not alter cell substructure or change enzyme activities. We strongly support the use of cell cultures for studying the cellular action(s) of amiodarone and offer conditions suitable for such experiments.

Original languageEnglish
Pages (from-to)1212-1216
Number of pages5
JournalIsrael Journal of Medical Sciences
Issue number12
StatePublished - Dec 1996
Externally publishedYes


  • ATP
  • Amiodarone
  • Contractile activity
  • Cultured cardiomyocytes
  • Electron microscopy
  • Enzymes
  • Membrane potentials


Dive into the research topics of 'Cardiomyocytes in culture - A model to study the cellular actions of amiodarone'. Together they form a unique fingerprint.

Cite this