Carcinogen‐Mediated Amplification of Specific DNA Sequences

Sara Lavi*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


A model experimental system based on SV40‐transformed Chinese hamster embryo cells and a highly sensitive in situ hybridization procedure was designed. Exposure of the cells to different categories of chemical and physical carcinogens resulted in the induction of SV40 DNA synthesis in the treated cells. Although the carcinogen‐mediated amplification of SV40 DNA sequences is regulated by the viral “A” gene, neither infectious virus nor complete viral DNA molecules were rescued from the treated cells. A heterogenous collection of DNA molecules containing SV40 sequences was generated following treatment with DMBA. Restriction enzyme analysis of the amplified DNA molecules in the Hirt supernatant revealed that not all sequences in the integrated SV40 inserts are present. The possibility that the amplification of SV40 sequences is a reflection of a general gene amplification phenomenon mediated by carcinogens is discussed.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalJournal of Cellular Biochemistry
Issue number2
StatePublished - 1982
Externally publishedYes


  • AFB
  • Chinese hamster
  • DBA
  • DMBA
  • DNA synthesis
  • EMS
  • MCA
  • MNNG
  • amplification
  • benzo(a)pyrene
  • carcinogenesis
  • chromosomal rearrangement
  • gene amplification tsA209
  • onion skin replication
  • origin of replication
  • phenanthrene
  • short‐term assay
  • transformation


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